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SARS-CoV-2 (2019-nCoV) Antigen Reagents

SARS-CoV-2 (2019-nCoV) Detection Kits, Antibodies and Proteins

The newly identified coronavirus, SARS-CoV-2 (2019-nCoV), has led to pneumonia (COVID-19) that sickened over 240,000 people worldwide. SARS-CoV-2 belongs to the Betacoronavirus Genus, which also includes SARS CoV (2003) and MERS CoV (2012). Same as all other coronaviruses, the genome of SARS-CoV-2 (2019-nCoV) encodes the spike protein, the envelope protein, the membrane protein, and the nucleocapsid protein.

The spike protein (S-protein) mediates receptor binding and membrane fusion. Spike protein contains two subunits, S1 and S2. S1 contains a receptor binding domain (RBD), which is responsible for recognizing and binding with the cell surface receptor. S2 subunit is the "stem" of the structure, which contains other basic elements needed for the membrane fusion. The spike protein is the common target for neutralizing antibodies and vaccines. It's been reported that SARS-CoV-2 (2019-nCoV) (ref.) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. Indeed, the recombinant Spike protein can bind with recombinant ACE2 protein.

The Nucleocapsid Protein (N-protein) is the most abundant protein in coronavirus. The N-protein is a highly immunogenic phosphoprotein, and it is normally very conserved. The N protein of coronavirus is often used as a marker in diagnostic assays.

To aid the efforts of developing diagnostic kits, vaccines and neutralizing antibodies against this virus, Sino Biological Inc. has developed a panel of research reagents for SARS-CoV-2, including recombinant antigens (the N protein, S protein, the S1 and S2 subunits of the S protein, and the RBD domain of the S proteins), antibodies, antigen detection kits and genes.

SARS-CoV-2 Antigens

 

SARS-CoV-2 Antibodies

 
N SARS-CoV-2 (2019-nCoV) Nucleocapsid-His Protein
[ Expresed in Insect cell ]
S1+S2 SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Protein
[ Expresed in Insect cell, bind with ACE2 ]
   
S1 SARS-CoV-2 (2019-nCoV) Spike S1-His Protein
[ Expresed in HEK293 cell, bind with ACE2 ]
S1 SARS-CoV-2 (2019-nCoV) Spike S1-mFc Protein
[ Expresed in HEK293 cell, bind with ACE2 ]
   
S1 SARS-CoV-2 (2019-nCoV) Spike S1-His Protein
[ Expresed in Insect cell, bind with ACE2 ]
S1 SARS-CoV-2 (2019-nCoV) Spike S1-Fc Protein
[ Expresed in HEK293 cell, bind with ACE2 ]
   
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Protein
[ Expresed in HEK293 cell, bind with ACE2 ]
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-His Protein
[ Expresed in Insect cell, bind with ACE2 ]
   
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-Fc Protein
[ Expresed in HEK293 cell, bind with ACE2 ]
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-His Protein
[ Expresed in HEK293 cell, bind with ACE2 ]
   
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-rFc Protein
[ Expresed in HEK293 cell, bind with ACE2 ]
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-His Protein,Biotinylated
[ Expresed in HEK293 cell ]
   
RBD SARS-CoV-2 (2019-nCoV) Spike RBD-His Protein, Biotinylated
[ Expresed in Insect cell ]
S2 SARS-CoV-2 (2019-nCoV) Spike S2 ECD-His Protein
[ Expresed in Insect cell ]
   
S2 SARS-CoV-2 (2019-nCoV) Spike S2-mFc Protein
[ Expresed in HEK293 cell ]
Plpro SARS-CoV-2 (2019-nCoV) Plpro-His protein
[ Expresed in E.coli cell ]

The S and N detection kits employ a standard sandwich ELISA, allowing rapid quantification of the N and S antigens.
• All antibodies used in the kits are in-house developed monoclonal antibodies, ensuring batch-to-batch consistency.
• These assays may be used to quantify antigens on the surface of SARS-CoV-2 and/or pseudovirus, as well as antigens expressed recombinantly.

2019-nCoV N Detection Kit
(KIT40588)
More about SARS-CoV-2 Antigen Detection Assay

Sino Biological and Nanommune (Irvine, California) jointly developed the coronavirus antigen array. These arrays are constructed by printing recombinant antigens on a nitrocellulose-coated membrane. It can analyze serum antibodies against 65 antigens from 23 types of viruses known to cause respiratory tract infections, including SARS-COV-2 and the other six coronaviruses. The array is specifically designed for high throughput serological surveillance studies.

HCoV-OC43 HCoV-229E SARS-CoV
HCoV-NL63 HCoV-HKU1 MERS-CoV
SARS-CoV-2 Influeza virus RSV
Rhinovirus Metapneumovirus Parainfluenza
   
Adenovirus    
Description   Species More
CoV Nucleocapsid
2019-nCoV
  2019-nCoV

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Vector
CoV spike
2019-nCoV
  2019-nCoV

Catalog
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Vector
CoV spike
2019-nCoV
  2019-nCoV

Catalog
Tag
Vector
CoV spike
2019-nCoV
  2019-nCoV

Catalog
Tag
Vector
CoV spike
2019-nCoV
  2019-nCoV

Catalog
Tag
Vector
CoV Hemagglutinin esterase   2019-nCoV

Catalog
Tag
Vector
CoV Membrane   2019-nCoV

Catalog
Tag
Vector
CoV 3CLpro / 3C-like protease   2019-nCoV

Catalog
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Vector
CoV NSP3   2019-nCoV

Catalog
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Vector
CoV Envelope   2019-nCoV

Catalog
Tag
Vector
CoV Hemagglutinin esterase   2019-nCoV

Catalog
Tag
Vector
 

Capacity of Viral Reagents Development

• 2009.5 Launched Recombinant Influenza A H5N1 Hemagglutinin Protein

• 2009.12 Launched Influenza A H5N1 HA ELISA Pair Set and H1N1 HA ELISA Pair Set

• 2012.12 Launched MERS-CoV Spike Protein(S1,S2,S-RBD),NP protein within 50 Days after Sequence Published.

• 2013.4 Completed R&D of H7N9 HA Protein & H7N9 NA Protein in 12 Days.

• 2013.10 Launched SARS CoV Spike Protein, NP Protein.

• 2014.2 Completed R&D of EBOV NP, GP, VP40, VP24 Proteins.

• 2016.6 Completed R&D of ZIKV Envelope, Membrane protein in 14 Days.

• 2020.1 Completed R&D of SARS-CoV-2 (2019-nCoV) Viral Proteins (Spike, S1, S2, RBD protein) in 12 Days.

View Related Viral Products

 

Reference

• Xintian Xu.Evolution of the novel coronavirus from the ongoing Wuhan outbreak and modeling of its spike protein for risk of human transmission.SCIENCE CHINA Life Sciences.2020
• Bo Diao, Zeqing Feng.Human Kidney is a Target for Novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection.medRxiv preprint