PD-1 ELISA Kit, Human

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PD-1 ELISA Kit, Human: General Information

Product name
PD-1 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
1.57 pg/mL
Assay range
3.91-250 pg/mL
Specificity
Recognizes both recombinant and natural Human PD-1
Interference
Preparations of the factors listed below at 10 ng/mL in a mid-range recombinant human PD1 control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 48.00 87.00 182.00
SD 1.65 8.08 7.77
CV(%) 3.4% 9.3% 4.3%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 49.00 101.00 191.00
SD 4.16 12.29 8.33
CV(%) 8.4% 12.2% 4.4%
Recovery
The recovery of Human PDCD1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
103
Range
100 -105%
Linearity
Serum
  Recovery of detected
1:1 92%
1:2 103%
1:4 97%
1:8 75%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This PD-1 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human PD-1 protein in Serum . It contains recombinant Human PD-1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

PD-1 ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human PD1. The factors listed below were prepared at 10 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

PD-1 ELISA Kit, Human: Alternative Names

CD279 ELISA Kit, Human; hPD-1 ELISA Kit, Human; hPD-l ELISA Kit, Human; hSLE1 ELISA Kit, Human; PD-1 ELISA Kit, Human; PD1 ELISA Kit, Human; SLEB2 ELISA Kit, Human

PD-1 Background Information

Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-1 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.
Full Name
programmed cell death 1
References
  • James ES, et al. (2005) PDCD1: a tissue-specific susceptibility locus for inherited inflammatory disorders. Genes Immun. 6(5): 430-7.
  • Okazaki T, et al. (2007) PD-1 and PD-1 ligands: from discovery to clinical application. Int Immunol. 19(7): 813-24.
  • del Rio ML, et al. (2008) PD-1/PD-L1, PD-1/PD-L2, and other co-inhibitory signaling pathways in transplantation. Transpl Int. 21(11): 1015-28.
  • Riley JL.(2009) PD-1 signaling in primary T cells. Immunol Rev. 229(1): 114-25.
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