Anti-IkB alpha Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-IkB alpha Magnetic Beads-IP Kit Product Components

Components Storage
Anti-IkB alpha Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-IkB alpha magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-IkB alpha Magnetic Beads-IP Kit Product Description

The Anti-IkB alpha magnetic Beads, conjugated with Anti-IkB alpha antibody, are used for immuneprecipitation (IP) of IkB alpha proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing IkB alpha proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound IkB alpha proteins are dissociated from the magnetic beads using an elution buffer.

Anti-IkB alpha Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-IkB alpha Antibody(12045-RP02)
Immunogen
Recombinant Human IkB alpha / NFKBIA protein (Catalog#12045-H07E)
Species Reactivity
Human IkB alpha / NFKBIA
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human IkB alpha / NFKBIA (rh IkB alpha / NFKBIA; Catalog#12045-H07E; Phe2-Leu317; NP_065390.1). IkB alpha / NFKBIA specific IgG was purified by Human IkB alpha / NFKBIA affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-IkB alpha Magnetic Beads Immunoprecipitation (IP) Kit: Alternative Names

Anti-IKBAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MAD-3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-NFKBIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

IkB alpha Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IkB alpha, NFKBIA, or IKBA), is a member of the NF-kappa-B inhibitor family that function to inhibit the NF-kB transcription factor. NFKBIA inhibits NF-kB by masking the nuclear localization signals (NLS) of NF-kB proteins and keeping them sequestered in an inactive state in the cytoplasm. In addition, NFKBIA blocks the ability of NF-κB transcription factors to bind to DNA, which is required for NF-kB's proper functioning. Signal-induced degradation of I kappa B alpha exposes the nuclear localization signal of NF-kappa B, thus allowing it to translocate into the nucleus and activate transcription from responsive genes. An autoregulatory loop is established when NF-kappa B induces expression of the I kappa B alpha gene and newly synthesized I kappa B alpha accumulates in the nucleus where it negatively regulates NF-kappa B-dependent transcription. As part of this post-induction repression, the nuclear export signal on I kappa B alpha mediates transport of NF-kappa B-I kappa B alpha complexes from the nucleus to the cytoplasm. Deletion of NFKBIA has an effect that is similar to the effect of EGFR amplification in the pathogenesis of glioblastoma and is associated with comparatively short survival. Polymorphisms in NFKBIA may be important in pre-disposition to and outcome after treatment, of multiple myeloma (MM). The NFKBIA gene product, IkappaBalpha, binds to NF-kappaB preventing its activation and is important in mediating resistance to apoptosis in B-cell lymphoproliferative diseases.
Full Name
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Research Areas
References
  • Verma IM, et al. (1995) Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation. Genes Dev. 9 (22): 2723-35.
  • Jacobs MD, et al. (1998) Structure of an IkappaBalpha/NF-kappaB complex. Cell 95 (6): 749-58.
  • Hay RT, et al. (1999) Control of NF-kappa B transcriptional activation by signal induced proteolysis of I kappa B alpha. Philos Trans R Soc Lond B Biol Sci. 354(1389): 1601-9.
  • Spink CF, et al. (2007) Haplotypic structure across the I kappa B alpha gene (NFKBIA) and association with multiple myeloma. Cancer Lett. 246(1-2): 92-9.
  • Bredel M, et al. (2011) NFKBIA deletion in glioblastomas. N Engl J Med. 364(7): 627-37.
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