IkB alpha ELISA Kit, Human

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IkB alpha ELISA Kit, Human: General Information

Product name
IkB alpha ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Lysate
Sensitivity
3.6 pg/mL
Assay range
12.5-800 pg/mL
Specificity
Recognizes both recombinant and natural Human IkB alpha
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 109.00 219.00 433.00
SD 2.31 10.87 12.71
CV(%) 2.1% 5.0% 2.9%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 157.40 261.20 509.20
SD 12.44 27.29 23.46
CV(%) 7.9% 10.4% 4.6%
Recovery
The recovery of Human NFKBIA spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=4)
Average % Recovery
99
Range
83-116%
Linearity
Supernatant
  Recovery of detected
1:2 93%
1:4 92%
1:8 98%
1:16 107%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This IkB alpha ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IkB alpha protein in Lysate . It contains recombinant Human IkB alpha, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

IkB alpha ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human NFKBIA. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

IkB alpha ELISA Kit, Human: Alternative Names

IKBA ELISA Kit, Human; MAD-3 ELISA Kit, Human; NFKBI ELISA Kit, Human

IkB alpha Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IkB alpha, NFKBIA, or IKBA), is a member of the NF-kappa-B inhibitor family that function to inhibit the NF-kB transcription factor. NFKBIA inhibits NF-kB by masking the nuclear localization signals (NLS) of NF-kB proteins and keeping them sequestered in an inactive state in the cytoplasm. In addition, NFKBIA blocks the ability of NF-κB transcription factors to bind to DNA, which is required for NF-kB's proper functioning. Signal-induced degradation of I kappa B alpha exposes the nuclear localization signal of NF-kappa B, thus allowing it to translocate into the nucleus and activate transcription from responsive genes. An autoregulatory loop is established when NF-kappa B induces expression of the I kappa B alpha gene and newly synthesized I kappa B alpha accumulates in the nucleus where it negatively regulates NF-kappa B-dependent transcription. As part of this post-induction repression, the nuclear export signal on I kappa B alpha mediates transport of NF-kappa B-I kappa B alpha complexes from the nucleus to the cytoplasm. Deletion of NFKBIA has an effect that is similar to the effect of EGFR amplification in the pathogenesis of glioblastoma and is associated with comparatively short survival. Polymorphisms in NFKBIA may be important in pre-disposition to and outcome after treatment, of multiple myeloma (MM). The NFKBIA gene product, IkappaBalpha, binds to NF-kappaB preventing its activation and is important in mediating resistance to apoptosis in B-cell lymphoproliferative diseases.
Full Name
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Research Areas
References
  • Verma IM, et al. (1995) Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation. Genes Dev. 9 (22): 2723-35.
  • Jacobs MD, et al. (1998) Structure of an IkappaBalpha/NF-kappaB complex. Cell 95 (6): 749-58.
  • Hay RT, et al. (1999) Control of NF-kappa B transcriptional activation by signal induced proteolysis of I kappa B alpha. Philos Trans R Soc Lond B Biol Sci. 354(1389): 1601-9.
  • Spink CF, et al. (2007) Haplotypic structure across the I kappa B alpha gene (NFKBIA) and association with multiple myeloma. Cancer Lett. 246(1-2): 92-9.
  • Bredel M, et al. (2011) NFKBIA deletion in glioblastomas. N Engl J Med. 364(7): 627-37.
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