GCSF Receptor / G-CSFR Protein

GCSF Receptor / G-CSFR Protein Overview

GCSF Receptor / G-CSFR reagents

Granulocyte colony-stimulating factor or CSF3 (138970), is a glycoprotein of MW 20,000-25,000 Da that is produced by macrophages stimulated with endotoxin. It plays an important role in granulopoiesis during the inflammatory process. CSF3 exerts its biologic effects through interaction with specific cell surface receptors. Binding studies with radiolabeled CSF3 indicated that CSF3 receptor is expressed not only by progenitor and mature neutrophilic granulocytes, but also by nonhematopoietic cells such as placental cells, endothelial cells, and various carcinoma cell lines. Fukunaga et al. (1990) cloned and characterized cDNAs for human CSF3 receptor. They found that it is abundantly expressed in the human placenta. The receptor contains 813 amino acids and shows marked homology (62.5%) with its murine counterpart. It was found to consist of extracellular, transmembrane, and cytoplasmic domains. Two other classes of the human CSF3 receptor were identified, one of which had a deletion of the transmembrane domain and seemed to represent a secreted, soluble receptor. The third class contained a 27-amino acid insertion in the cytoplasmic domain and was highly expressed in the placenta. Seto et al. (1992) found that CSF3R is subdivided into several regions: an Ig-like domain, a cytokine receptor homologous domain, 3 fibronectin type III domains, a transmembrane domain, and a cytoplasmic region. No canonical 'TATA' box was found upstream of the cap site. About 110 bp upstream of the transcription initiation site, an 18-bp element was found that is homologous to sequences found in the promoter of human myeloperoxidase (606989) and neutrophil elastase (130120) genes.

GCSF Receptor / G-CSFR protein family

Belongs to the type I cytokine receptor family. Type 2 subfamily.

GCSF Receptor / G-CSFR protein name

Recommended name
Granulocyte colony-stimulating factor receptor
Short name
G-CSF receptor||G-CSF-R
Aliases
GCSFR
Alternative name
CD antigen CD114

GCSF Receptor / G-CSFR Gene family protein

GCSF Receptor / G-CSFR Protein Sequence

Species Human GCSF Receptor / G-CSFR protein
Length 836
Mass (Da) 92156
Sequence Human GCSF Receptor / G-CSFR protein sequence
Species Mouse GCSF Receptor / G-CSFR protein
Length 837
Mass (Da) 93379
Sequence Mouse GCSF Receptor / G-CSFR protein sequence
Species
Length
Mass (Da)
Sequence

GCSF Receptor / G-CSFR Protein Molecular Weight & PI

Granulocyte colony-stimulating factor receptor precursor (G-CSF receptor) (G-CSF-R) (CD114 antigen) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 25-836 Granulocyte colony-stimulating factor

Molecular weight (Da)

89618.33

Theoretical pI

5.76

GCSF Receptor / G-CSFR Protein Structure

HOMOLOGY-BUILT MODEL OF THE CYTOKINE RECEPTOR HOMOLOGY TITLE 2 DOMAIN OF HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR TITLE 3 RECEPTOR
Deposition Author(s)
D.SMITH,H.TREUTLEIN
Experimental Data Snapshot
1AZ7 From PDB

Human GCSF Receptor / G-CSFR protein Secondary structure

GCSF Receptor / G-CSFR Protein Interaction

Recombinant GCSF Receptor / G-CSFR Protein Feature

GCSF Receptor / G-CSFR Protein, Human, Recombinant (Fc Tag)

High Purity
> 95 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Measured by its ability to inhibit GCSF-induced proliferation of NFS60 mouse myeloid cells.The ED50 for this ettect is typically 2-20 ng/ml in the presence of 0.125ng/ml of recombinant human GCSF.

Recombinant GCSF Receptor / G-CSFR protein citations

Title
Characterization of Stress-Exposed Granulocyte Colony Stimulating Factor Using ELISA and Hydrogen/Deuterium Exchange Mass Spectrometry
Year
2014
Author
Tsuchida, D;Yamazaki, K;Akashi, S;
Journal
J. Am. Soc. Mass Spectrom.

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