Anti-PD-1 Magnetic Beads Immunoprecipitation (IP) Kit

Price:
Size:
Number:

Anti-PD-1 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-PD-1 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-PD-1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-PD-1 Magnetic Beads-IP Kit Product Description

The Anti-PD-1 magnetic Beads, conjugated with Anti-PD-1 antibody, are used for immuneprecipitation (IP) of PD-1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing PD-1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound PD-1 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-PD-1 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-PD-1 Antibody(108583-T36)
Immunogen
A synthetic peptide corresponding to the center region of the Cynomolgus PD-1/PDCD1
Species Reactivity
Cynomolgus PD-1/PDCD1
Source
Polyclonal Cynomolgus Rabbit IgG
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Cynomolgus PD-1/PDCD1, and purified by antigen affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

PD-1 Background Information

Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-1 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.
Full Name
programmed cell death 1
References
  • James ES, et al. (2005) PDCD1: a tissue-specific susceptibility locus for inherited inflammatory disorders. Genes Immun. 6(5): 430-7.
  • Okazaki T, et al. (2007) PD-1 and PD-1 ligands: from discovery to clinical application. Int Immunol. 19(7): 813-24.
  • del Rio ML, et al. (2008) PD-1/PD-L1, PD-1/PD-L2, and other co-inhibitory signaling pathways in transplantation. Transpl Int. 21(11): 1015-28.
  • Riley JL.(2009) PD-1 signaling in primary T cells. Immunol Rev. 229(1): 114-25.
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait