TNF-alpha Protein, Human, Recombinant

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TNF-alpha Protein, Human, Recombinant: Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.
The ED50 for this effect is typically 5-50 pg/mL.
Protein Construction
A DNA sequence encoding the human TNF-α soluble form (NP_000585.2) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus.
Accession#
Expressed Host
E. coli
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human TNF-α consists of 158 amino acids and has a predicted molecular mass of 17.4 kDa which is also estimated by SDS-PAGE.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TNF-alpha Protein, Human, Recombinant: Images

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 5-50 pg/mL.

TNF-alpha Protein, Human, Recombinant: Alternative Names

DIF Protein, Human; TNF-alpha Protein, Human; TNFA Protein, Human; TNFSF2 Protein, Human

TNF-alpha Background Information

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD12a; p55/6) and TNF-R2 (TNF receptor type 2; CD12b; p75/8), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.
Full Name
tumor necrosis factor
Research Areas
References
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
  • Houttuynia cordata blocks HSV infection through inhibition of NF-κB activation
    Author
    Chen, X;Wang, Z;Yang, Z;Wang, J;Xu, Y;Tan, RX;Li, E;
    Year
    2011
    Journal
    Antiviral Res.
    Application
    cell-based (culture)
  • Inhibition of herpes simplex virus infection by oligomeric stilbenoids through ROS generation
    Author
    Chen, X;Qiao, H;Liu, T;Yang, Z;Xu, L;Xu, Y;Ge, HM;Tan, RX;Li, E;
    Year
    2012
    Journal
    Antiviral Res.
    Application
    cell-based
  • Production of bioactive, SUMO-modified, and native-like TNF-α of the rhesus monkey, Macaca mulatta, in Escherichia coli
    Author
    Jia, D;Yang, H;Wan, L;Cheng, J;Lu, X;
    Year
    2012
    Journal
    Appl. Microbiol. Biotechnol.
    Application
    Cytotoxicity assay
  • A laboratory evaluation of medicinal herbs used in china for the treatment of hand, foot, and mouth disease
    Author
    Chen, X;Wang, C;Xu, L;Chen, X;Wang, W;Yang, G;Tan, RX;Li, E;Jin, Y;
    Year
    2013
    Journal
    Evid Based Complement Alternat Med
  • Increased sCD200 Levels in Vitreous of Patients With Proliferative Diabetic Retinopathy and Its Correlation With VEGF and Proinflammatory Cytokines
    Author
    Xu, Y;Cheng, Q;Yang, B;Yu, S;Xu, F;Lu, L;Liang, X;
    Year
    2015
    Journal
    Invest. Ophthalmol. Vis. Sci.
  • CYLD Promotes TNF-α-Induced Cell Necrosis Mediated by RIP-1 in Human Lung Cancer Cells
    Author
    Lin, X;Chen, Q;Huang, C;Xu, X;
    Year
    2016
    Journal
    Mediators Inflamm.
    Application
    cell-based
  • Neutralization of TNFα in tumor with a novel nanobody potentiates paclitaxel-therapy and inhibits metastasis in breast cancer
    Author
    Ji, X;Peng, Z;Li, X;Yan, Z;Yang, Y;Qiao, Z;Liu, Y;
    Year
    2016
    Journal
    Cancer Lett.
    Application
    cell-based
  • Human S100A7 Induces Mature Interleukin1α Expression by RAGE-p38 MAPK-Calpain1 Pathway in Psoriasis
    Author
    Lei, H;Li, X;Jing, B;Xu, H;Wu, Y;
    Year
    2017
    Journal
    PLoS ONE
    Application
    cell-based
  • Purification of target proteins from intracellular inclusions mediated by intein cleavable polyhydroxyalkanoate synthase fusions
    Author
    Du, J;Rehm, BHA;
    Year
    2017
    Journal
    Microb. Cell Fact.
    Application
    Elisa;target protein
  • T0001, a variant of TNFR2-Fc fusion protein, exhibits improved Fc effector functions through increased binding to membrane-bound TNFα
    Author
    Shen, Y;Li, G;Gu, C;Chen, B;Chen, A;Li, H;Gao, B;Liang, C;Wu, J;Yang, T;Jin, L;Su, Y;
    Year
    2017
    Journal
    PLoS ONE
    Application
    FACS
  • Transcription factor NF-kappa B represses ANT1 transcription and leads to mitochondrial dysfunctions
    Author
    Zhang, C;Jiang, H;Wang, P;Liu, H;Sun, X;
    Year
    2017
    Journal
    Sci Rep
    Application
    cell-based
  • TGF-α Overexpression in Breast Cancer Bone Metastasis and Primary Lesions and TGF-α Enhancement of Expression of Procancer Metastasis Cytokines in Bone Marrow Mesenchymal Stem Cells
    Author
    Sun, J;Cui, H;Gao, Y;Pan, Y;Zhou, K;Huang, J;Lan, J;Wei, Q;Liu, X;Liu, L;Xian, C;
    Year
    2018
    Journal
    BioMed Research International
    Application
    cell-based
  • Downregulation of tumor suppressor RACK1 by Helicobacter pylori infection promotes gastric carcinogenesis through the integrin β-1/NF-κB signaling pathway
    Author
    Hu, Y;Liu, J;Li, X;Cai, Y;He, C;Li, N;Xie, C;Xiong, Z;Ge, Z;Lu, N;Zhu, Y;
    Year
    2019
    Journal
    Cancer Letters
    Application
    cell-based
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