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High amplification efficiency
Wide linear range
A Single Nucleotide Polymorphism (SNP) is a DNA sequence variation occurring commonly within a population in which a single nucleotide — A, T, C or G — in the genome differs between members of a biological species or paired chromosomes. For example, two sequenced DNA fragments from different individuals, ATGCGTA to ATGCATA, contain a difference in a single nucleotide.
The main applications of SNP analysis by qPCR include:
• Gene mapping
• Human disease analysis
• Individuation of medical treatment
• Crop and animal breeding
Basic principle of SNP method
High Resolution Melt (HRM) analysis is a new gene analysis technique based on melting curve of different forms because of different single nucleotide melting temperature. SNP is analyzed through HRM.
Advantages of HRM
• Simple operation
• Short analysis time
• High sensitivity and specificity
• Lower cost
• Accurate results
• High throughput
• Do not use specific probes
• Do not limit check sites
• No pollution because of operation of closed tubes
HRM method&Taqman probes method of SNP
Human CA12 gene HRM analysis
Normalized and Sifted Melting curve of three different standards
Normalized and Temp-Sifted Difference Plot of three different standards
Accepted Sample Types
a tissue(≥100 mg); b in vitro cultured cell (≥106 cells);c genomic DNA(≥5 µg)
DNA preparation, PCR, HRM analysis
HRM curve, gene mutation and SNP analysis results.
A normal qPCR experiment is completed in 2-3 weeks. Time of particular cases are in line with genes and samples detected.
Please consult our company for detailed information.
Expression of CCNA1 in muscle of patients with facioscapulohumeral muscle dystrophy is altered, and its protein modulates the expression of vascular emdothelial growth factor and promotes hormone-dependent growth and angiogenesis of breast cancer. So rs565998602 locus of CCNA1 of some cell lines was analyzed by HRM.
Normalized and Sifted Melting curve of CCNA1 SNP analysis
Normalized and temp-shifted difference plot of CCNA1 SNP analysis