CD146/MCAM: CD146/MCAM Protein | CD146/MCAM Antibody

CD146/MCAM Gene family

CD146/MCAM Background

The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body, and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumours, and thus has been suggested to play an important role in tumour progression and the development of metastasis in certain human carcinomas.

Reference for CD146/MCAM

  • Mills L, et al. (2002) Fully human antibodies to MCAM/MUC18 inhibit tumor growth and metastasis of human melanoma. Cancer Res. 62(17): 5106-14.
  • Taira E, et al. (2004) Characterization of Gicerin/MUC18/CD146 in the rat nervous system. J Cell Physiol. 198(3): 377-87.
  • Fritzsche FR, et al. (2008) CD146 protein in prostate cancer: revisited with two different antibodies. Pathology. 40(5): 457-64.
  • Bidlingmaier S, et al. (2009) Identification of MCAM/CD146 as the target antigen of a human monoclonal antibody that recognizes both epithelioid and sarcomatoid types of mesothelioma. Cancer Res. 69(4): 1570-7.
  • Boneberg EM, et al. (2009) Soluble CD146 is generated by ectodomain shedding of membrane CD146 in a calcium-induced, matrix metalloprotease-dependent process. Microvasc Res. 78(3): 325-31.

CD146/MCAM Protein

CD146/MCAM protein function

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. {ECO:0000269|PubMed:11036077, ECO:0000269|PubMed:8292890}.

CD146/MCAM protein expression

Tissue specificity

Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.

CD146/MCAM protein sequence

This sequence information is just for reference only.From Uniport

  • Length
  • Mass (Da)

CD146/MCAM Antibody

There are 21 CD146/MCAM antibodies which are validated in multiple tissues with various applications, including FCM, ELISA, ELISA(Cap), WB, IHC-P, ELISA(Det), ICC/IF. There are 10 CD146/MCAM antibody for FCM, 10 CD146/MCAM antibody for ELISA, 1 CD146/MCAM antibody for ELISA(Cap), 4 CD146/MCAM antibody for WB, 6 CD146/MCAM antibody for IHC-P, 1 CD146/MCAM antibody for ELISA(Det), 3 CD146/MCAM antibody for ICC/IF. Among all these CD146/MCAM antibodies, there are 5 anti-CD146/MCAM mouse monoclonal antibodies , 12 anti-CD146/MCAM rabbit monoclonal antibodies , 4 anti-CD146/MCAM rabbit polyclonal antibodies . All the CD146/MCAM anbodies are produced in house and all are in stock. CD146/MCAM antibody customerized service is available.

CD146/MCAM Gene

MCAM gene / cDNA is a protein-coding gene which located on 11q23.3. The MCAM gene is conserved in chimpanzee, dog, mouse, rat, chicken, zebrafish, and frog.212 organisms have orthologs with human gene MCAM.