PD-L1 Protein, Mouse, Recombinant (His Tag)

1/1
Price:
Size:
Number:

PD-L1 Protein, Mouse, Recombinant (His Tag): Product Information

Purity
> 98 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PD-L1 at 1 μg/ml (100 μl/well) can bind mouse PD1 with a linear range of 6.25-400 ng/ml.
Protein Construction
A DNA sequence encoding the mouse CD274 (NP_068693.1) extracellular domain (Met 1-Thr 238) was fused with a polyhistidine tag at the C-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
Phe 19
Molecule Mass
The secreted recombinant mouse CD274 comprises 231 amino acids and has a predicted molecular mass of 26.3 kDa. As a result of glycosylation, it migrates as an approximately 40-45 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PD-L1 Protein, Mouse, Recombinant (His Tag): Images

PD-L1 Protein, Mouse, Recombinant (His Tag): Alternative Names

A530045L16Rik Protein, Mouse; B7h1 Protein, Mouse; Pdcd1l1 Protein, Mouse; Pdcd1lg1 Protein, Mouse; Pdl1 Protein, Mouse

PD-L1 Background Information

Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.
Full Name
CD274 molecule
References
  • Iwai Y, et al. (2002) Involvement of PD-L1 on tumor cells in the escape from host immune system and tumor immunotherapy by PD-L1 blockade. Proc Natl Acad Sci U S A. 99(19): 12293-7.
  • Ghebeh H, et al. (2006) The B7-H1 (PD-L1) T lymphocyte-inhibitory molecule is expressed in breast cancer patients with infiltrating ductal carcinoma: correlation with important high-risk prognostic factors. Neoplasia. 8(3): 190-8.
  • Salih HR, et al. (2006) The role of leukemia-derived B7-H1 (PD-L1) in tumor-T-cell interactions in humans. Exp Hematol. 34(7): 888-94.
  • Wilcox RA, et al. (2009) B7-H1 (PD-L1, CD274) suppresses host immunity in T-cell lymphoproliferative disorders. Blood. 114(10): 2149-58.
  • Ruggiero A, et al. (2009) Crystal structure of PD-L1, a ribosome inactivating protein from Phytolacca dioica L. leaves with the property to induce DNA cleavage. Biopolymers. 91(12): 1135-42.
  • Soluble production of a biologically active single-chain antibody against murine PD-L1 in< i> Escherichia coli</i>
    Author
    JJ Drees, LB Augustin, MJ Mertensotto…
    Year
    2014
    Journal
    Protein Expression and Purification
    Application
    antigen/binding
  • Non-invasive assessment of murine PD-L1 levels in syngeneic tumor models by nuclear imaging with nanobody tracers
    Author
    Broos, K;Keyaerts, M;Lecocq, Q;Renmans, D;Nguyen, T;Escors, D;Liston, A;Raes, G;Breckpot, K;Devoogdt, N;
    Year
    2017
    Journal
    Oncotarget
    Application
    ELISA; ELISA; SPR
  • PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 Pathways
    Author
    Zhao, Y;Lee, CK;Lin, CH;Gassen, RB;Xu, X;Huang, Z;Xiao, C;Bonorino, C;Lu, LF;Bui, JD;Hui, E;
    Year
    2019
    Journal
    Immunity
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait