PD-1 Antibody

PD-1 Antibody Overview

There are 39 PD-1 antibodies which are validated in multiple tissues with various applications, including Neutralization, FCM, ELISA(Cap), ELISA, IHC-P, ELISA(Det), WB, IP. There are 3 PD-1 antibody for Neutralization, 14 PD-1 antibody for FCM, 2 PD-1 antibody for ELISA(Cap), 18 PD-1 antibody for ELISA, 6 PD-1 antibody for IHC-P, 1 PD-1 antibody for ELISA(Det), 2 PD-1 antibody for WB, 2 PD-1 antibody for IP. Among all these PD-1 antibodies, there are 2 anti-PD-1 , 24 anti-PD-1 mouse monoclonal antibodies , 4 anti-PD-1 rabbit monoclonal antibodies , 9 anti-PD-1 rabbit polyclonal antibodies . All the PD-1 anbodies are produced in house and all are in stock. PD-1 antibody customerized service is available.

PD-1 Antibody ELISA

PD-1 Antibody western blot

PD-1 Antibody western blot Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Cynomolgus PD-1/PDCD1, and purified by antigen affinity chromatography.

PD-1 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

PD-1 Antibody flow cytometry

PD-1 Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PD1/PDCD1/CD279 (rh PD1/PDCD1/CD279; Catalog#10377-H08H; NP_005009.2; Met1-Gln167). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

PD-1 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

PD-1 Antibody immunohistochemistry

PD-1 Antibody immunohistochemistry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PD1 / PDCD1 / CD279 (rh PD1 / PDCD1 / CD279; Catalog#10377-H08H; NP_005009.2; Met1-Gln167). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

PD-1 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:50-1:200
Notes  

PD-1 Antibody immunoprecipitation

PD-1 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Canine PD1/PDCD1/CD279 ( Catalog#70109-D08H; XP_543338.3; Met1-Leu169). PD1/PDCD1/CD279 specific IgG was purified by Canine PD1/PDCD1/CD279 affinity chromatography.

PD-1 Antibody immunoprecipitation Validated

Application IP
Dilution IP:0.5-2 μL/mg of lysate
Notes  

PD-1 Antibody Neutralization

PD-1 Antibody Neutralization Preparation

PD-1 Antibody Neutralization Validated

Application Neutralization
Dilution The neutralization activity of PD1 Neutralizing Antibody is Measured by its ability to neutralize PD1_PD-L1 pathway mediated inhibition of IFN-gamma secretion in the Mixed lymphocyte Reaction.
Notes  

PD-1 antibody citations

Title
Enhancement of SIV-specific cell mediated immune responses by co-administration of soluble PD-1 and Tim-3 as molecular adjuvants in mice
Year
2014
Author
Lijun Xiao1,2, Dimin Wang3, Pingchao Li1, Yi Jin1,2, Liqiang Feng1*, Caijun Sun1*, and Ling Chen
Journal
Human Vaccines & Immunotherapeutics
Title
Non-conventional Inhibitory CD4+Foxp3-PD-1hi T Cells as a Biomarker of Immune Checkpoint Blockade Activity
Year
2018
Author
Zappasodi, R;Budhu, S;Hellmann, MD;Postow, MA;Senbabaoglu, Y;Manne, S;Gasmi, B;Liu, C;Zhong, H;Li, Y;Huang, AC;Hirschhorn-Cymerman, D;Panageas, KS;Wherry, EJ;Merghoub, T;Wolchok, JD;
Journal
Cancer Cell