NGF / NGFB Neutralizing Antibody


NGF / NGFB Neutralizing Antibody (Rabbit Monoclonal antibody) General Information

Product name
NGF / NGFB Neutralizing Antibody
Validated applications
Species reactivity
Reacts with: Human
Human NGF
Has cross-reactivity with Mouse β-NGF (Catalog # 50385-MNAC) in ELISA assay
Recombinant Human NGF / NGFB Protein (Catalog#11050-HNAC)
This antibody was obtained from a rabbit immunized with purified, recombinant Human NGF / NGFB (rh NGF / NGFB; Catalog#11050-HNAC; NP_002497.2; Ser122-Arg239) and was produced using recombinant antibody technology.
Monoclonal Rabbit IgG Clone #R711
Protein A
0.2 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0
< 3 EU/mg
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

NGF / NGFB Neutralizing Antibody (Rabbit Monoclonal antibody) Validated Applications

Block: In a functional ELISA which immobilized recombinant Human β-NGF (Catalog# 11050-HNAC) at 2μg/mL (100 µL/well) in the plate, the Rabbit anti-Human β-NGF Monoclonal Antibody (Catalog# 11050-R711) can block the binding of 5 μg/mL of biotinylated Human NTRK1/Fc Chimera Protein (Catalog# 11073-H03H-B) to human β-NGF, the EC50 is 3.84 μg/mL.
Neutralization: The neutralization activity of antibody is Measured by its ability to neutralize β­NGF­induced proliferation in the TF­1 human erythroleukemic cell line. The Neutralization titer (IC50) is typically 0.048­0.19 µg/mL in the presence of 10 ng/mL Recombinant Human β­NGF.
Please Note: Optimal concentrations/dilutions should be determined by the end user.

NGF / NGFB Neutralizing Antibody (Rabbit Monoclonal antibody) Images

Cell Proliferation Induced by β­NGF was Neutralized by Human β­NGF Antibody. Recombinant Human β­NGF (Catalog 11050-HNAC) stimulates proliferation in the TF­1 human erythroleukemic cell line. Proliferation elicited by Recombinant Human β­NGF (10 ng/mL) is neutralized by increasing concentrations of Rabbit Anti­Human β­NGF Monoclonal Antibody (Catalog 11050-R711). The IC50 is typically 0.048­0.19 µg/mL.

NGF / NGFB Neutralizing Antibody: Alternative Names

Anti-Beta-NGF Antibody; Anti-HSAN5 Antibody; Anti-NGFB Antibody

NGF Background Information

Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In addition, previous studies indicated that NGF may also have an important role in the regulation of the immune system.
Full Name
nerve growth factor (beta polypeptide)
  • Castellanos MR, et al. (2003) Evaluation of the neurorestorative effects of the murine beta-nerve growth factor infusions in old rat with cognitive deficit. Biochem Biophys Res Commun. 312(4): 867-72.
  • Wang TH, et al. (2008) Effects of pcDNA3-beta-NGF gene-modified BMSC on the rat model of Parkinson's disease. J Mol Neurosci. 35(2): 161-9.
  • Perrard MH, et al. (2009) Redundancy of the effect of TGFbeta1 and beta-NGF on the second meiotic division of rat spermatocytes. Microsc Res Tech. 72(8): 596-602.

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Mouse MAb

Rabbit PAb

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