LIGHT Protein

LIGHT Protein Overview

LIGHT reagents

Viruses gain entry into cells by means of receptors encoded by genes of the host cell. Herpes simplex virus (HSV) has been shown to infect cells, including activated T lymphocytes, by means of an HSV envelope glycoprotein D attaching to the herpesvirus entry mediator (HVEM), a member of the tumor necrosis factor receptor superfamily (TNFRSF14; 602746). By screening activated T cells by FACS for staining with a surrogate receptor composed of the extracellular domain of HVEM and the Fc of IgG (see 146790), Mauri et al. (1998) determined by competition experiments that HVEM binds with secreted lymphotoxin A (LTA; 153440) and a 29- to 30-kD protein that they designated LIGHT (for homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes). By screening an activated T-cell library, they identified a LIGHT cDNA, which encodes a 240-amino acid protein with a 37-amino acid N-terminal cytosolic domain and a 22-amino acid type II transmembrane stretch. In the C-terminal receptor-binding domain, LIGHT exhibits 34% identity with lymphotoxin B (LTB; 600978) and 31% identity with FASL (TNFSF6; 134638) and lesser identity with other TNFSFs. The residues with the greatest homology are those forming the beta-strand scaffold. Northern blot analysis revealed expression of a 2.5-kb transcript for LIGHT predominantly in spleen but also in brain where a secondary 3.5-kb transcript was seen. Weaker expression was detected in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression was detected in fetal tissues, endocrine glands, or nonhematopoietic tumor lines. By screening soluble forms of novel TNFSF proteins derived from an EST database with an HVEM-FcIgG fusion protein, Harrop et al. (1998) also identified TNFSF14, which they termed HVEML. By RT-PCR analysis, Granger et al. (2001) detected a smaller LIGHT transcript with an internal deletion of 36 amino acids that removes the entire transmembrane domain of the type II glycoprotein. Immunoprecipitation analysis showed cytosolic expression of a 28-kD protein corresponding to this alternative transcript of LIGHT. Glycosidase treatment and immunoprecipitation analysis indicated that the full-length form of LIGHT, but not the shorter variant, is glycosylated. A shed form of LIGHT was capable of binding HVEM but was not capable of inducing apoptosis. Granger et al. (2001) concluded that LIGHT exists in several molecular forms that are directed to the extracellular space, the cell membrane, and cytosolic compartments.

LIGHT protein family

Belongs to the tumor necrosis factor family.

LIGHT protein name

Recommended name
Tumor necrosis factor ligand superfamily member 14
Aliases
CD258, HVEM-L, LIGHT, LTg
Alternative name
Herpes virus entry mediator ligand CD_antigen: CD258 Tumor necrosis factor ligand superfamily member 14, membrane form Tumor necrosis factor ligand superfamily member 14, soluble form

LIGHT Protein Sequence

Species Human LIGHT protein
Length 240
Mass (Da) 26350
Sequence Human LIGHT protein sequence
Species Mouse LIGHT protein
Length 239
Mass (Da) 26338
Sequence Mouse LIGHT protein sequence
Species
Length
Mass (Da)
Sequence

LIGHT Protein Molecular Weight & PI

Tumor necrosis factor ligand superfamily member 14 (Herpes virus entry mediator ligand) (HVEM-L) (Herpesvirus entry mediator ligand) (CD258 antigen) [Contains: Tumor necrosis factor ligand superfamily member 14, membrane form; Tumor necrosis factor ligand superfamily member 14, soluble form] Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 1-240 Tumor necrosis factor ligand superfamily

Molecular weight (Da)

26350.27

Theoretical pI

9.09

LIGHT Protein Structure

Crystal structure of the light and hvem complex
Deposited
2014-11-11   Released:  2015-02-04
Deposition Author(s)
Liu, W., Ramagoal, U.A., Himmel, D., Bonanno, J.B., Nathenson, S.G., Almo, S.C., Atoms-to-Animals: The Immune Function Network (IFN), New York Structural Genomics Research Consortium (NYSGRC)
Organism(s)
Homo sapiens
Expression System
Drosophila
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.3000 Å
R-Value Free
0.231
R-Value Work
0.188
4RSU From PDB

Human LIGHT protein Secondary structure

LIGHT Protein Interaction

Recombinant LIGHT Protein Feature

LIGHT Protein, Human, Recombinant (Fc Tag)

High Purity
> (83.4+7.9) % as determined by SDS-PAGE.
Low Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.

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