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Anti-MICA Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-MICA Magnetic Beads-IP Kit Product Components

Components Storage
Anti-MICA Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-MICA magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-MICA Magnetic Beads-IP Kit Product Description

The Anti-MICA magnetic Beads, conjugated with Anti-MICA antibody, are used for immuneprecipitation (IP) of MICA proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing MICA proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound MICA proteins are dissociated from the magnetic beads using an elution buffer.

Anti-MICA Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-MICA Antibody(12302-RP02)
Immunogen
Recombinant Human MICA protein (Catalog#12302-H08H)
Species Reactivity
Human MICA
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human MICA (rh MICA; Catalog#12302-H08H; AAH16929.1; Met 1-Gln 308). MICA specific IgG was purified by Human MICA affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-MICA Magnetic Beads Immunoprecipitation (IP) Kit: Alternative Names

Anti-DAMA-345G11.2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-FLJ36918ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-FLJ60820ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MGC111087ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MGC21250ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MIC-AALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PERB11.1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

MICA Background Information

MHC class I chain-related molecules A (MICA) is one of the genes in the HLA class I region, which belongs to MHC class I family. It is the member of the non-classical class I family that displays the greatest degree of polymorphism. The MICA protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. The Natural killer group 2D (NKG2D), a C-type lectin-like activating immunoreceptor, is a receptor of MICA, which was detected on most gammadelta T cells, CD8+ alphabeta T cells, and natural killer (NK) cells. Effector cells from all these subsets could be stimulated by ligation of NKG2D. Engagement of NKG2D activated cytolytic responses of gammadelta T cells and NK cells against transfectants and epithelial tumor cells expressing MICA. The MICA system is a novel, avidin-free immunohistochemical detection system that provides a significant increase in sensitivity compared to traditional immunodetection systems.
Full Name
MHC class I polypeptide-related sequence A
References
  • Choy MK, et al. (2010) MICA polymorphism: biology and importance in immunity and disease. Trends Mol Med. 16(3): 97-106.
  • Li J, et al. (2005) Distinct pattern of human Vdelta1 gammadelta T cells recognizing MICA. Cell Mol Immunol. 2(4): 253-8.
  • Mangham DC, et al. (2000) MICA-a highly sensitive and avidin-free immunohistochemical detection system. Adv Anat Pathol. 7(6): 360-4.
  • Bauer S, et al. (1999) Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science. 285(5428): 727-9.
  • Groh V, et al. (1998) Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells. Science. 279: 1737-40.
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