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Anti-ICAM-1 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-ICAM-1 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-ICAM-1 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-ICAM-1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-ICAM-1 Magnetic Beads-IP Kit Product Description

The Anti-ICAM-1 magnetic Beads, conjugated with Anti-ICAM-1 antibody, are used for immuneprecipitation (IP) of ICAM-1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing ICAM-1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound ICAM-1 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-ICAM-1 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-ICAM-1 Antibody(80022-T60)
Immunogen
Recombinant Rat ICAM-1/CD54 Protein (Catalog#80022-R08H)
Species Reactivity
Rat ICAM-1/CD54
Source
Polyclonal Rat Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Rat ICAM-1/CD54 (rR ICAM-1/CD54; Catalog#80022-R08H; Q00238-1; Met1-Thr493). ICAM-1/CD54 specific IgG was purified by affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

ICAM-1 Background Information

Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 9 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and in animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for a number of immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.
Full Name
intercellular adhesion molecule 1
References
  • Xu H, et al. (2001) The role of ICAM-1 molecule in the migration of Langerhans cells in the skin and regional lymph node. Eur J Immunol. 31(10): 3085-93.
  • Terol MJ, et al. (2003) Soluble intercellular adhesion molecule-1 (s-ICAM-1/s-CD54) in diffuse large B-cell lymphoma: association with clinical characteristics and outcome. Ann Oncol. 14(3): 467-74.
  • Mendez MP, et al. (2006) Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury. Am J Physiol Lung Cell Mol Physiol. 290(5): L962-70.
  • Lawson C, et al. (2009) ICAM-1 signaling in endothelial cells. Pharmacol Rep. 61(1): 22-32.
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