Anti-CD40 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-CD40 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-CD40 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-CD40 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-CD40 Magnetic Beads-IP Kit Product Description

The Anti-CD40 magnetic Beads, conjugated with Anti-CD40 antibody, are used for immuneprecipitation (IP) of CD40 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD40 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD40 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-CD40 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-CD40 Antibody(101510-T36)
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human CD40 / TNFRSF5
Species Reactivity
Human CD40 / TNFRSF5
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human CD40 / TNFRSF5, and purified by antigen affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-CD40 Magnetic Beads Immunoprecipitation (IP) Kit: Alternative Names

Anti-Bp50ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-CDW40ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-p50ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TNFRSF5ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

CD40 Background Information

CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.
Full Name
CD40 molecule, TNF receptor superfamily member 5
References
  • van Kooten C, et al. (2000). CD40-CD40 ligand. J Leukoc Biol. 67 (1): 2-17.
  • Bhushan A, et al. (2002). CD40:CD40L interactions in X-linked and non-X-linked hyper-IgM syndromes. Immunol Res. 24 (3): 311-24.
  • Chatzigeorgiou A, et al. (2009) CD40/CD40L signaling and its implication in health and disease. Biofactors. 35(6): 474-83.
  • Li R, et al. (2009) Expression of CD40 and CD40L in Gastric Cancer Tissue and Its Clinical Significance. Int J Mol Sci. 10(9): 3900-17.
  • Lievens D, et al. (2009) The multi-functionality of CD40L and its receptor CD40 in atherosclerosis. Thromb Haemost. 102(2): 206-14.
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