Anti-CD155 / PVR Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-CD155 / PVR Magnetic Beads-IP Kit Product Components

Components Storage
Anti-CD155 / PVR Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-CD155 / PVR magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-CD155 / PVR Magnetic Beads-IP Kit Product Description

The Anti-CD155 / PVR magnetic Beads, conjugated with Anti-CD155 / PVR antibody, are used for immuneprecipitation (IP) of CD155 / PVR proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD155 / PVR proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD155 / PVR proteins are dissociated from the magnetic beads using an elution buffer.

Anti-CD155 / PVR Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-CD155 / PVR Antibody(10109-RP02)
Immunogen
Recombinant Human CD155 protein (Catalog#10109-H08H)
Species Reactivity
Human CD155 / PVR / NECL-5
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CD155 isoform a (rh CD155; Catalog#10109-H08H; NP_006496.2; Met 1-Asn 343). CD155 specific IgG was purified by human CD155 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-CD155 / PVR Magnetic Beads Immunoprecipitation (IP) Kit: Alternative Names

Anti-CD155ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-HVEDALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-Necl-5ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-NECL5ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PVSALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TAGE4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

CD155 / PVR Background Information

CD155, commonly known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5), is a type I transmembrane single-span glycoprotein, and belongs to the nectins and nectin-like (Necl) subfamily. CD155 was originally identified based on its ability to mediate the cell attachment and entry of poliovirus (PV), an etiologic agent of the central nervous system disease poliomyelitis. The normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. CD155 may assist in an efficient humoral immune response generated within the intestinal immune system. It has been demonstrated that CD155 can be recognized and bond by DNAM-1 and CD96 which promote the adhension, migration and NK-cell killing, and thus efficiently prime cell-mediated tumor-specific immunity.
Full Name
poliovirus receptor
References
  • Freistadt MS, et al. (2000) Hematopoietic cells from CD155-transgenic mice express CD155 and support poliovirus replication ex vivo. Microb Pathog. 29(4): 203-12.
  • Sato T, et al. (2004) Involvement of heterophilic trans-interaction of Necl-5/Tage4/PVR/CD155 with nectin-3 in formation of nectin- and cadherin-based adherens junctions. Genes Cells. 9(9): 791-9.
  • Kakunaga S, et al. (2004) Enhancement of serum- and platelet-derived growth factor-induced cell proliferation by Necl-5/Tage4/poliovirus receptor/CD155 through the Ras-Raf-MEK-ERK signaling. J Biol Chem. 279(35): 36419-25.
  • Sato T, et al. (2005) Common signaling pathway is used by the trans-interaction of Necl-5/Tage4/PVR/CD155 and nectin, and of nectin and nectin during the formation of cell-cell adhesion. Cancer Sci. 96(9): 578-89.
  • Minami Y, et al. (2007) Involvement of up-regulated Necl-5/Tage4/PVR/CD155 in the loss of contact inhibition in transformed NIH3T3 cells. Biochem Biophys Res Commun. 352(4): 856-60.
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