Anti-ICAM-1 Antibody (Rabbit Monoclonal antibody) General Information
Reacts with: Mouse
Recombinant Mouse ICAM-1 / CD54 Protein (Catalog#50440-M08H)
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse ICAM-1 / CD54 (rM ICAM-1 / CD54; Catalog#50440-M08H; NP_034623.1; Met1-Asn485).
Monoclonal Rabbit IgG Clone #009
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Flow cytometric analysis of Mouse ICAM1(CD54) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse ICAM1(CD54), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence staining of Mouse ICAM1 in Raw264.7 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-mouse ICAM1 monoclonal antibody (dilution ratio 1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Cell membrane.
Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 9 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and in animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for a number of immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.
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