Human SARS Coronavirus Spike S1 Subunit Antibody, Rabbit PAb, Antigen Affinity Purified

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Human SARS Coronavirus Spike S1 Subunit Antibody, Rabbit PAb, Antigen Affinity Purified (Rabbit Polyclonal antibody) General Information

Product name
Human SARS Coronavirus Spike S1 Subunit Antibody, Rabbit PAb, Antigen Affinity Purified
Validated applications
WB,ELISA,IHC-P,FCM,ICC/IF,IP (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Application notes
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
SARS CoV spike
Immunogen
Recombinant Human SARS Coronavirus Spike S1 Subunit protein (Catalog#40150-V08B1)
Preparation
Produced in rabbits immunized with purified, recombinant Human SARS Coronavirus Spike S1 Subunit (Catalog#40150-V08B1; AAX16192.1; Met1-Arg667). Human SARS Coronavirus Spike S1 Subunit specific IgG was purified by Human SARS Coronavirus Spike S1 Subunit affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Human SARS Coronavirus Spike S1 Subunit Antibody, Rabbit PAb, Antigen Affinity Purified (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Human SARS Coronavirus Spike S1 Subunit Antibody, Rabbit PAb, Antigen Affinity Purified (Rabbit Polyclonal antibody) Images

Anti-Human SARS Coronavirus Spike S1 Subunit rabbit polyclonal antibody at 1:1000 dilution

Sample:Human SARS Coronavirus Spike S1 Subunit Recombinant Protein

Lane A: 30ng

Secondary

Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/1000 dilution.

Performed under reducing conditions.

Human SARS Coronavirus Spike S1 Subunit Antibody, Rabbit PAb, Antigen Affinity Purified: Alternative Names

Anti-S1 Antibody

CoV spike Background Information

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell: they are essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. The SARS-CoV spike (S) protein is composed of two subunits; the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV.
References
  • Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.
  • Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.
  • Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.

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