c-Kit ELISA Kit, Human

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c-Kit ELISA Kit, Human: General Information

Product name
c-Kit ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
9.4 pg/mL
Assay range
39-2500 pg/mL
Specificity
Recognizes both recombinant and natural Human c-Kit
Interference
Preparations of FLT3、MCSFR、PDGFRα、PDGFRβ、VEGFR1、VEGFR2 and VEGFR3 at 25 ng/mL in a mid-range recombinant human KIT control were assayed for interference. No significant interference was observed.Recombinant human SCF does not cross-react in this assay but does interfere at concentrations greater than 5 ng/mL.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.36 0.70 1.29
SD 0.01 0.02 0.03
CV(%) 2.0% 3.0% 2.7%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.35 0.66 1.42
SD 0.02 0.07 0.12
CV(%) 6.3% 9.9% 8.6%
Recovery
The recovery of Human KIT spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
122.537166852924
Range
116%-127%
Linearity
Serum
  Recovery of detected
1:2 115%
1:4 84%
1:8 81%
1:16 121%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This c-Kit ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human c-Kit protein in Serum . It contains recombinant Human c-Kit, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

c-Kit ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human KIT. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
Preparations of FLT3、MCSFR、PDGFRα、PDGFRβ、VEGFR1、VEGFR2 and VEGFR3 at 25 ng/mL in a mid-range recombinant human KIT control were assayed for interference. No significant interference was observed.Recombinant human SCF does not cross-react in this assay but does interfere at concentrations greater than 5 ng/mL.

c-Kit ELISA Kit, Human: Alternative Names

C-Kit ELISA Kit, Human; CD117 ELISA Kit, Human; PBT ELISA Kit, Human; SCFR ELISA Kit, Human

c-Kit Background Information

C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains.and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family and CSF-1/PDGF receptor subfamily. C-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. C-Kit has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumour in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumours and in distinguishing seminomas from embryonal carcinomas. Mutations in c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.
Full Name
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
References
  • Andre C, et al. (1997) Sequence analysis of two genomic regions containing the KIT and the FMS receptor tyrosine kinase genes. Genomics. 39(2):216-26.
  • Yarden Y, et al. (1987) Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. EMBO J. 6(11):3341-51.
  • Leong KG, et al. (2008) Generation of a prostate from a single adult stem cell. Nature. 456(7223): 804-8.
  • Edling CE, et al. (2007) c-Kit--a hematopoietic cell essential receptor tyrosine kinase. Int J Biochem Cell Biol. 39(11):1995-8.
  • McIntyre A, et al. (2005) Amplification and overexpression of the KIT gene is associated with progression in the seminoma subtype of testicular germ cell tumors of adolescents and adults. Cancer Res. 65(18):8085-9.
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