FCGRT & B2M Heterodimer Protein, Human, Recombinant (His & AVI Tag), Biotinylated

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FCGRT & B2M Heterodimer Protein, Human, Recombinant (His & AVI Tag), Biotinylated: Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Biotin/Protein Ratio
0.5-1 as determined by the HABA assay.
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the extracellular domain (Met1-Ser297) of human FCGRT (NP_004098.1) was fused with a C-terminal polyhistidine tagged AVI tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human B2M (NP_004039.1) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the human FCGRT/B2M heterodimer was purified. The purified heterodimer was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Ala 24 & Ile 21
Molecule Mass
The recombinant heterodimer of human FCGRT/B2M comprises 399 (300+99) amino acids and has a calculated molecular mass of 45.3 (33.6+11.7) KDa.
Formulation
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FCGRT & B2M Heterodimer Protein, Human, Recombinant (His & AVI Tag), Biotinylated: Images

FCGRT & B2M Heterodimer Protein, Human, Recombinant (His & AVI Tag), Biotinylated: Alternative Names

alpha-chain Protein, Human; FCRN Protein, Human

FCGRT & B2M Heterodimer Background Information

Full Name
Fc fragment of IgG, receptor, transporter, alpha
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