EGFR Protein

EGFR Protein Overview

EGFR reagents

EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development (Wang et al., 2004).

EGFR protein family

Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.

EGFR protein name

Recommended name
Epidermal growth factor receptor
Aliases
ERBB1, erb-b2 receptor tyrosine kinase 1, erythroblastic leukemia viral (v-erb-b) oncogene homolog (avian)
Alternative name
Proto-oncogene c-ErbB-1 Receptor tyrosine-protein kinase erbB-1

EGFR Gene family protein

Erb-b2 receptor tyrosine kinases

EGFR Protein Sequence

Species Human EGFR protein
Length 1210
Mass (Da) 134277
Sequence Human EGFR protein sequence
Species Mouse EGFR protein
Length 1210
Mass (Da) 134853
Sequence Mouse EGFR protein sequence
Species
Length
Mass (Da)
Sequence

EGFR Protein Molecular Weight & PI

Epidermal growth factor receptor precursor (EC 2.7.10.1) (Proto-oncogene c-ErbB-1) (Receptor tyrosine-protein kinase erbB-1) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 25-1210 Epidermal growth factor receptor.

Molecular weight (Da)

132012.67

Theoretical pI

6.17

EGFR Protein Structure

Crystal structure of isolated domain III of the extracellular region of the epidermal growth factor receptor in complex with the Fab fragment of IMC-11F8
Deposited
2007-10-19   Released:  2008-02-19
Deposition Author(s)
Ferguson, K.M., Li, S., Kussie, P.
Organism(s)
Homo sapiens
Expression System
Mus musculus, Spodoptera frugiperda
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.5800 Å
R-Value Free
0.291
R-Value Work
0.227
3B2U From PDB

Human EGFR protein Secondary structure

EGFR Protein Interaction

Recombinant EGFR Protein Feature

EGFR Protein, Human, Recombinant (aa 668-1210, His & GST Tag)

High Purity
> 75 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
High Activity
The specific activity was determined to be>70 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate

Recombinant EGFR protein citations

Title
Chimeric antigen receptor
Year
2012
Author
H Shiku, Y Orito, J Mineno, S Okamoto…
Journal
US 20140242701 A1
Title
Generating bispecific human IgG1 and IgG2 antibodies from any antibody pair
Year
2012
Author
P Strop, WH Ho, LM Boustany, YN Abdiche…
Journal
J. Mol. Biol.
Title
Phage-derived fully human antibody scFv fragment directed against human vascular endothelial growth factor receptor 2 blocked its interaction with VEGF
Year
2012
Author
Zhang, J;Li, H;Wang, X;Qi, H;Miao, X;Zhang, T;Chen, G;Wang, M;
Journal
Biotechnol. Prog.
Title
Phage‐derived fully human antibody scFv fragment directed against human vascular endothelial growth factor receptor 2 blocked its interaction with VEGF
Year
2012
Author
J Zhang, H Li, X Wang, H Qi, X Miao…
Journal
Biotechnol. Prog
Title
Self-assembling complexes of quantum dots and scFv antibodies for cancer cell targeting and imaging
Year
2012
Author
Zdobnova, TA;Stremovskiy, OA;Lebedenko, EN;Deyev, SM;
Journal
PLoS ONE
Title
Affinity capture of biotinylated proteins at acidic conditions to facilitate hydrogen/deuterium exchange mass spectrometry analysis of multimeric protein complexes
Year
2013
Author
Jensen, PF;Jørgensen, TJ;Koefoed, K;Nygaard, F;Sen, JW;
Journal
Anal. Chem.
Title
Development of a cell-based assay measuring the activation of FcγRIIa for the characterization of therapeutic monoclonal antibodies
Year
2014
Author
Tada, M;Ishii-Watabe, A;Suzuki, T;Kawasaki, N;
Journal
PLoS ONE
Title
Endogenous antibody responsiveness to epidermal growth factor receptor is associated with immunoglobulin allotypes and overall survival of patients with …
Year
2014
Author
JP Pandey, E Kistner-Griffin…
Journal
Neuro-Oncology
Title
Selection of DNA aptamers against epidermal growth factor receptor with high affinity and specificity
Year
2014
Author
DL Wang, YL Song, Z Zhu, XL Li, Y Zou…
Journal
Biochemical and Biophysical Research Communications
Title
(64)Cu-Labeled Gp2 Domain for PET Imaging of Epidermal Growth Factor Receptor
Year
2016
Author
Kruziki, MA;Case, BA;Chan, JY;Zudock, EJ;Woldring, DR;Yee, D;Hackel, BJ;
Journal
Mol. Pharm.
Title
Covalent and Oriented Surface Immobilization of Antibody Using Photoactivatable Antibody Fc-Binding Protein Expressed in Escherichia coli
Year
2016
Author
Lee, Y;Jeong, J;Lee, G;Moon, JH;Lee, MK;
Journal
Anal. Chem.
Title
Intramolecular trimerization, a novel strategy for making multispecific antibodies with controlled orientation of the antigen binding domains
Year
2016
Author
Alvarez-Cienfuegos, A;Nuñez-Prado, N;Compte, M;Cuesta, AM;Blanco-Toribio, A;Harwood, SL;Villate, M;Merino, N;Bonet, J;Navarro, R;Muñoz-Briones, C;Sørensen, KM;Mølgaard, K;Oliva, B;Sanz, L;Blanco, FJ;Alvarez-Vallina, L;
Journal
Sci Rep
Title
RN1, a novel galectin-3 inhibitor, inhibits pancreatic cancer cell growth in vitro and in vivo via blocking galectin-3 associated signaling pathways
Year
2016
Author
Zhang, L;Wang, P;Qin, Y;Cong, Q;Shao, C;Du, Z;Ni, X;Li, P;Ding, K;
Journal
Oncogene
Title
Conditional internalization of PEGylated nanomedicines by PEG engagers for triple negative breast cancer therapy
Year
2017
Author
Su, YC;Burnouf, PA;Chuang, KH;Chen, BM;Cheng, TL;Roffler, SR;
Journal
Nat Commun
Title
Development of a series of novel 4-anlinoquinazoline derivatives possessing quinazoline skeleton: Design, synthesis, EGFR kinase inhibitory efficacy, and evaluation of anticancer activities in vitro
Year
2017
Author
Chang, J;Ren, H;Zhao, M;Chong, Y;Zhao, W;He, Y;Zhao, Y;Zhang, H;Qi, C;
Journal
Eur J Med Chem
Title
Dual Electrochemiluminescence Signal System for In Situ and Simultaneous Evaluation of Multiple Cell-Surface Receptors.
Year
2017
Author
Zhou, B;Qiu, Y;Wen, Q;Zhu, M;Yang, P;
Journal
ACS Appl Mater Interfaces
Title
EGFR induces DNA decomposition via phosphodiester bond cleavage
Year
2017
Author
Tong, Y;Li, S;Huang, C;
Journal
Sci Rep
Title
Plasma-Generated OH Radical Production for Analyzing Three-Dimensional Structure in Protein Therapeutics
Year
2017
Author
Minkoff, BB;Blatz, JM;Choudhury, FA;Benjamin, D;Shohet, JL;Sussman, MR;
Journal
Sci Rep
Title
Rotundarpene inhibits TNF-伪-induced activation of the Akt, mTOR, and NF-魏B pathways, and the JNK and p38 associated with production of reactive oxygen species
Year
2017
Author
Kim, A;Nam, YJ;Shin, YK;Lee, MS;Sohn, DS;Lee, CS;
Journal
Mol. Cell. Biochem.
Title
Tumor detection using magnetosome nanoparticles functionalized with a newly screened EGFR/HER2 targeting peptide
Year
2017
Author
Xiang, Z;Yang, X;Xu, J;Lai, W;Wang, Z;Hu, Z;Tian, J;
Journal
Biomaterials
Title
Tumor detection using magnetosome nanoparticles functionalized with a newly screened EGFR/HER2 targeting peptide
Year
2017
Author
Xiang, Z;Yang, X;Xu, J;Lai, W;Wang, Z;Hu, Z;Tian, J;
Journal
Biomaterials
Title
Expression and purification of the human epidermal growth factor receptor extracellular domain
Year
2017
Author
Wang, L;Yan, J;Yan, J;Xu, H;Zhang, D;Wang, X;Sheng, J;
Journal
Protein Expr. Purif.
Title
Near infrared fluorescence imaging of EGFR expression in vivo using IRDye800CW-nimotuzumab
Year
2017
Author
Bernhard, W;El-Sayed, A;Barreto, K;Gonzalez, C;Hill, W;Parada, A;Fonge, H;Geyer, C;
Journal
Oncotarget
Title
6,7-Dimorpholinoalkoxy quinazoline derivatives as potent EGFR inhibitors with enhanced antiproliferative activities against tumor cells
Year
2018
Author
Zhang, Y;Chen, L;Xu, H;Li, X;Zhao, L;Wang, W;Li, B;Zhang, X;
Journal
Eur J Med Chem
Title
Application of the Open qPCR Instrument for the in Vitro Selection of DNA Aptamers against Epidermal Growth Factor Receptor and Drosophila C Virus
Year
2018
Author
Damase, TR;Miura, TA;Parent, CE;Allen, PB;
Journal
ACS Comb Sci
Title
Aptamer-drug conjugate: targeted delivery of doxorubicin in a HER3 aptamer-functionalized liposomal delivery system reduces cardiotoxicity
Year
2018
Author
Dou, XQ;Wang, H;Zhang, J;Wang, F;Xu, GL;Xu, CC;Xu, HH;Xiang, SS;Fu, J;Song, HF;
Journal
Int J Nanomedicine
Title
Dsg2 via Src-mediated transactivation shapes EGFR signaling towards cell adhesion
Year
2018
Author
Ungewiß, H;Rötzer, V;Meir, M;Fey, C;Diefenbacher, M;Schlegel, N;Waschke, J;
Journal
Cell. Mol. Life Sci.
Title
Label-free real-time ultrasensitive monitoring of non-small cell lung cancer cell interaction with drugs
Year
2018
Author
Dai, H;Jiao, Y;Sun, Z;Cao, Z;Chen, X;
Journal
Biomed. Opt. Express
Title
Real-time quartz crystal microbalance cytosensor based on a signal recovery strategy for in-situ and continuous monitoring of multiple cell membrane glycoproteins
Year
2018
Author
Zhou, B;Hao, Y;Long, D;Yang, P;
Journal
Biosensors and Bioelectronics
Title
Synthesis and Characterization of EGFR-Targeted Immunoporphysomes
Year
2018
Author
Qiu, Y;
Journal
Thesis
Title
The Immunogenicity and Anti-Tumor Efficacy of a Rationally Designed EGFR Vaccine
Year
2018
Author
Cheng, C;Deng, L;Li, R;
Journal
Cell. Physiol. Biochem.
Title
Automated literature mining and hypothesis generation through a network of Medical Subject Headings
Year
2018
Author
Wilson, S;Wilkins, A;Holt, M;Choi, B;Konecki, D;Lin, C;Koire, A;Chen, Y;Kim, S;Wang, Y;Wastuwidyaningtyas, B;Qin, J;Donehower, L;Lichtarge, O;
Journal
bioRxiv
Title
A comparative analysis of EGFR-targeting antibodies for gold nanoparticle CT imaging of lung cancer
Year
2018
Author
Ashton, JR;Gottlin, EB;Patz, EF;West, JL;Badea, CT;
Journal
PLoS ONE
Title
Targeting neratinib-induced diarrhea with budesonide and colesevelam in a rat model
Year
2018
Author
Secombe, KR;Ball, IA;Shirren, J;Wignall, AD;Finnie, J;Keefe, D;Avogadri-Connors, F;Olek, E;Martin, D;Moran, S;Bowen, JM;
Journal
Cancer Chemother. Pharmacol.

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