EGF Protein

EGF Protein Overview

EGF reagents

The epidermal growth factor (EGF; 131530) repeat motif defines a superfamily of diverse proteins involved in regulating a variety of cellular and physiologic processes. This motif features a series of conserved cysteines and glycines positioned in a domain of 30 to 40 residues. EGF-like repeat family members are predominantly secreted or cell surface molecules, often involved in the regulation of cell cycle, proliferation, and developmental processes.

EGF protein name

Recommended name
Pro-epidermal growth factor
Short name
EGF
Alternative name
Epidermal growth factor

EGF Protein Sequence

Species Human EGF protein
Length 1207
Mass (Da) 133994
Sequence Human EGF protein sequence
Species Mouse EGF protein
Length 1217
Mass (Da) 133072
Sequence Mouse EGF protein sequence
Species Rat EGF protein
Length 1133
Mass (Da) 124126
Sequence Rat EGF protein sequence

EGF Protein Molecular Weight & PI

Pro-epidermal growth factor precursor (EGF) [Contains: Epidermal growth factor (Urogastrone)] Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 23-1207 Pro-epidermal growth factor.

Molecular weight (Da)

131620.17

Theoretical pI

5.50

EGF Protein Structure

Crystal Structure of Human Epidermal Growth Factor
Deposited
2001-07-16   Released:  2001-10-24
Deposition Author(s)
Lu, H.S., Chai, J.J., Li, M., Huang, B.R., He, C.H., Bi, R.C.
Organism(s)
Homo sapiens
Expression System
Saccharomyces cerevisiae
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
3.0000 Å
R-Value Free
0.283
R-Value Work
0.231
1JL9 From PDB

Human EGF protein Secondary structure

EGF Protein Interaction

Recombinant EGF Protein Feature

EGF Protein, Human, Recombinant

High Purity
> 92 % as determined by SDS-PAGE
Low Endotoxin
Please contact us for more information.
High Activity
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.15-0.75 ng/mL.

Recombinant EGF protein citations

Title
Prokaryotic expression and refolding of EGFR extracellular domain and generation of phage display human scFv against EGFR
Year
2013
Author
Zhou, Y;Zhang, J;Jin, H;Chen, Z;Wu, Q;Li, W;Yue, M;Luo, C;Wang, M;
Journal
Biomed. Pharmacother.
Title
Expression and characterization of a soluble VEGF receptor 2 protein
Year
2014
Author
Liu, W;Zhang, X;Song, C;Bao, S;Lai, D;Mou, J;Jiang, T;Wang, N;
Journal
Cell Biosci
Title
miR-27a suppresses EV71 replication by directly targeting EGFR
Year
2014
Author
L Zhang, X Chen, Y Shi, B Zhou, C Du, Y Liu, S Han…
Journal
Virus genes
Title
Developing long-term in vitro microculture for neuropathology modelling
Year
2016
Author
Ramstad, OH;
Journal
Thesis
Title
Generation of Human Lens Epithelial-Like Cells from Patient-Specific Induced Pluripotent Stem Cells
Year
2016
Author
Li, D;Qiu, X;Yang, J;Liu, T;Luo, Y;Lu, Y;
Journal
J. Cell. Physiol.
Title
Cancer stem-like cell related protein CD166 degrades through E3 ubiquitin ligase CHIP in head and neck cancer
Year
2017
Author
Xiao, M;Yan, M;Zhang, J;Xu, Q;Qi, S;Wang, X;Chen, W;
Journal
Exp. Cell Res
Title
Plasma-Generated OH Radical Production for Analyzing Three-Dimensional Structure in Protein Therapeutics
Year
2017
Author
Minkoff, BB;Blatz, JM;Choudhury, FA;Benjamin, D;Shohet, JL;Sussman, MR;
Journal
Sci Rep
Title
Rotundarpene inhibits TNF-α-induced activation of the Akt, mTOR, and NF-κB pathways, and the JNK and p38 associated with production of reactive oxygen species
Year
2017
Author
Kits, E;
Journal
Product
Title
Expression and purification of the human epidermal growth factor receptor extracellular domain
Year
2017
Author
Wang, L;Yan, J;Yan, J;Xu, H;Zhang, D;Wang, X;Sheng, J;
Journal
Protein Expr. Purif.
Title
Androgen Promotes Differentiation of PLZF+ Spermatogonia pool via Indirect Regulatory Pattern
Year
2018
Author
Wang, J;Li, J;Gu, Y;Xia, Q;Song, W;Zhang, X;Yang, Y;Wang, W;Li, H;Zou, K;
Journal
bioRxiv
Title
OTUB2 Promotes Cancer Metastasis via Hippo-Independent Activation of YAP and TAZ
Year
2018
Author
Zhang, Z;Du, J;Wang, S;Shao, L;Jin, K;Li, F;Wei, B;Ding, W;Fu, P;van Dam, H;Wang, A;Jin, J;Ding, C;Yang, B;Zheng, M;Feng, XH;Guan, KL;Zhang, L;
Journal
Mol. Cell

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