Factor H Matched ELISA Antibody Pair Set,Human

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Factor H Matched ELISA Antibody Pair Set,Human General Information

Product name
Factor H Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human Factor H
Assay range
46.88-3000 pg/mL
Materials provided
1. Capture Antibody 0.5 mg/mL of mouse anti-Human Complement Factor H / CFH monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10714-MM02)
2. Detection Antibody 0.2 mg/mL of mouse anti-Human Complement Factor H / CFH monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10714-MM03)
3. Standard Each vial contains 38 ng of recombinant Human Complement Factor H / CFH. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ immediately (in 15 mins) in a manual defrost freezer, otherwise the value could decrease significantly. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3000 pg/mL is recommended.
Product overview
This Factor H Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human Factor H . It contains Human Factor H capture antibody, Human Factor H detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Factor H protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

Factor H Matched ELISA Antibody Pair Set,Human Images

Factor H Matched ELISA Antibody Pair Set,Human: Alternative Names

AHUS1 Matched ELISA Antibody Pair Set, Human; AMBP1 Matched ELISA Antibody Pair Set, Human; ARMD4 Matched ELISA Antibody Pair Set, Human; ARMS1 Matched ELISA Antibody Pair Set, Human; CFHL3 Matched ELISA Antibody Pair Set, Human; FH Matched ELISA Antibody Pair Set, Human; FHL1 Matched ELISA Antibody Pair Set, Human; HF Matched ELISA Antibody Pair Set, Human; HF1 Matched ELISA Antibody Pair Set, Human; HF2 Matched ELISA Antibody Pair Set, Human; HUS Matched ELISA Antibody Pair Set, Human

Factor H Background Information

Complement factor H, also known as H factor 1, and CFH, is a sialic acid containing glycoprotein that plays an integral role in the regulation of the complement-mediated immune system that is involved in microbial defense, immune complex processing, and programmed cell death. Factor H protects host cells from injury resulting from unrestrained complement activation. CFH regulates complement activation on self cells by possessing both cofactor activity for the Factor I mediated C3b cleavage, and decay accelerating activity against the alternative pathway C3 convertase, C3bBb. CFH protects self cells from complement activation but not bacteria/viruses. Due to the central role that CFH plays in the regulation of complement, there are many clinical implications arrising from aberrant CFH activity. Mutations in the Factor H gene are associated with severe and diverse diseases including the rare renal disorders hemolytic uremic syndrome (HUS) and membranoproliferative glomerulonephritis (MPGN) also termed dense deposit disease (DDD), membranoproliferative glomuleronephritis type II or dense deposit disease, as well as the more frequent retinal disease age related macular degeneration (AMD). In addition to its complement regulatory activities, factor H has multiple physiological activities and 1) acts as an extracellular matrix component, 2) binds to cellular receptors of the integrin type, and 3) interacts with a wide selection of ligands, such as the C-reactive protein, thrombospondin, bone sialoprotein, osteopontin, and heparin.
Full Name
complement factor H
References
  • Zipfel PF. (2001) Complement factor H: physiology and pathophysiology. Semin Thromb Hemost. 27(3): 191-9.
  • Zipfel PF, et al. (2008) The complement fitness factor H: role in human diseases and for immune escape of pathogens, like pneumococci. Vaccine. 26 Suppl 8: I67-74.
  • Ferreira VP, et al. (2010) Complement control protein factor H: the good, the bad, and the inadequate. Mol Immunol. 47(13): 2187-97.
  • Donoso LA, et al. (2010) The role of complement Factor H in age-related macular degeneration: a review. Surv Ophthalmol. 55(3): 227-46.
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