Anti-C5a Antibody

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Anti-C5a Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-C5a Antibody
Validated applications
IHC-P
Species reactivity
Reacts with: Human
Specificity
Human C5a
Has cross-reactivity in ELISA with
Human A2M
Immunogen
Recombinant Human Complement C5a protein (Catalog#10604-HNAE)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Complement C5a (rh Complement C5a; Catalog#10604-HNAE; CO5_HUMAN; Leu679-Arg751).
Source
Monoclonal Rabbit IgG Clone #215
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-C5a Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution
IHC-P 1:100-1:500
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-C5a Antibody (Rabbit Monoclonal antibody) Images

Immunochemical staining of human C5a in human cirrhosis with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).

Anti-C5a Antibody: Alternative Names

Anti-C5a Antibody; Anti-C5b Antibody; Anti-C5D Antibody; Anti-C5Da Antibody; Anti-Complement 5a Antibody; Anti-CPAMD4 Antibody; Anti-ECLZB Antibody

C5a Background Information

C5a is a protein fragment released from complement component C5. This 74 amino acid peptide in humans is generated by the cleavage of C5a convertase on the C5 α-chain during the classical, alternative, and lectin pathways of complement activation. The structure of C5a includes a core region consisting of four, anti-parallel alpha-helices held together by three disulfide linkages and a structured C-terminal tail, and C5a is rapidly metabolised by carboxypeptidase B to a 73 amino acid low activity form, C5a des-Arg. C5a is an extremely potent proinflammatory mediator, as well as a potent chemotactic factor for neutrophils and other leukocytes. It causes histamine release, increases in vascular permeability, induces several cytokines production from leukocytes, enhances neutrophil-endothelial cell adhesion, and augments the humoral and cell-mediated immune response. C5a is quickly metabolised by carboxypeptidases, forming the less potent C5adesArg. Acting via a classical G protein-coupled receptor, CD88, C5a and C5adesArg exert a number of effects essential to the innate immune response, while their actions at the more recently discovered non-G protein-coupled receptor, C5L2 (or GPR77), remain unclear. The widespread expression of C5a receptors throughout the body allows C5a to elicit a broad range of effects. Thus, C5a has been found to be a significant pathogenic driver in a number of immuno-inflammatory diseases, making C5a inhibition an attractive therapeutic strategy. C5a is a strong chemoattractant and is involved in the recruitment of inflammatory cells such as neutrophils, eosinophils, monocytes, and T lymphocytes, in activation of phagocytic cells and release of granule-based enzymes and generation of oxidants, all of which may contribute to innate immune functions or tissue damage. Accordingly, the anaphylatoxin C5a is implicated in a variety of diseases such as rheumatoid arthritis, systemic lupus erythematosus, reperfusion injury, Alzheimer's disease, and sepsis.
Full Name
complement component 5
References
  • Guo RF, et al.. (2005) Role of C5a in inflammatory responses. Annu Rev Immunol. 23: 821-52.
  • Guo RF, et al. (2006) C5a, a therapeutic target in sepsis. Recent Pat Antiinfect Drug Discov. 1(1): 57-65.
  • Manthey HD, et al. (2009) Complement component 5a (C5a). Int J Biochem Cell Biol. 41(11): 2114-7.

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