Human cytomegalovirus (HCMV) Glycoprotein B / gB Antibody, Mouse MAb

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Human cytomegalovirus (HCMV) Glycoprotein B / gB Antibody, Mouse MAb (Mouse Monoclonal antibody) General Information

Product name
Human cytomegalovirus (HCMV) Glycoprotein B / gB Antibody, Mouse MAb
Validated applications
WB,ELISA,IHC-P,FCM,ICC/IF,IP (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Application notes
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
CMV CMV Glycoprotein B
Immunogen
Recombinant Human CMV gB / Glycoprotein B protein (Catalog#10202-V08H1)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CMV gB (Catalog#10202-V08H1; AAA45920.1) extracellular domain (Met 1-Lys 700) linked with the cytoplasmic domain (Arg 777-Val 907). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #11B10D3E4
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Human cytomegalovirus (HCMV) Glycoprotein B / gB Antibody, Mouse MAb (Mouse Monoclonal antibody) Validated Applications

Application Dilution
WB 1:1000-1:5000
ELISA 1:1000-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Human cytomegalovirus (HCMV) Glycoprotein B / gB Antibody, Mouse MAb (Mouse Monoclonal antibody) Images

Anti-Human cytomegalovirus (HCMV) Glycoprotein B / gB mouse monoclonal antibody at 1:1000

Sample:Human cytomegalovirus (HCMV) Glycoprotein B / gB

Lane A: 100ng

Lane B: 20ng

Lane C: 4ng

Secondary

Rabbit Anti-Mouse IgGF( ab )2/HRP at 1:1000 dilution.

Performed under reducing conditions.

CMV Glycoprotein B Background Information

Cytomegalovirus (CMV) (human herpesvirus 5) glycoprotein B, also referred as CMV gB or gB, which belongs to the herpesviridae glycoprotein B family. It is a 97-amino acid glycoprotein encoded by the ORF of UL55. Cytomegalovirus Glycoprotein B protein is the most abundant component of the envelope, a target of neutralizing antibodies with at least two defined neutralizing epitopes and an essential replication component. Cytomegalovirus Glycoprotein B protein plays important roles in HCMV entry, cell-cell spread of internal virions, and fusion of infected cells. In addition, Cytomegalovirus Glycoprotein B protein is one envelope protein capable of heparin binding. It forms a physical association with host cell annexin II independent of the presence of calcium.
References
  • Lopper M,et al. (2002). Disulfide bond configuration of human cytomegalovirus glycoprotein B. J Virol. 76(12): 6073-82.
  • Isaacson MK, et al. (2009) Human cytomegalovirus glycoprotein B is required for virus entry and cell-to-cell spread but not for virion attachment, assembly, or egress. J Virol. 83(8): 3891-903.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

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Mouse MAb

Rabbit PAb

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