Anti-CD86 Antibody

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Anti-CD86 Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-CD86 Antibody
Validated applications
FCM
Species reactivity
Reacts with: Human
Specificity
Human CD86
Immunogen
Recombinant Human CD86 / B7-2 protein (Catalog#10699-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD86 / B7-2 (rh CD86 / B7-2; Catalog#10699-H08H; NP_008820.2; Met1-His239). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #06
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD86 Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD86 Antibody (Mouse Monoclonal antibody) Images

Flow cytometric analysis of Human B7-2(CD86) expression on Daudi cells. Cells were stained with purified anti-Human B7-2(CD86), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Anti-CD86 Antibody: Alternative Names

Anti-B7-2 Antibody; Anti-B7.2 Antibody; Anti-B70 Antibody; Anti-CD28LG2 Antibody; Anti-LAB72 Antibody

CD86 Background Information

CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B7), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. Recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.
Full Name
CD86 antigen
References
  • Chen YQ, et al. (2006) CD28/CTLA-4--CD80/CD86 and ICOS--B7RP-1 costimulatory pathway in bronchial asthma. Allergy. 61(1): 15-26.
  • Rau FC, et al. (2009) B7-1/2 (CD80/CD86) direct signaling to B cells enhances IgG secretion. J Immunol. 183(12): 7661-71.
  • Dai ZS, et al. (2009) Defective expression and modulation of B7-2/CD86 on B cells in B cell chronic lymphocytic leukemia. Int J Hematol. 89(5): 656-63.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

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Mouse MAb

Rabbit PAb

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