Anti-CD28 Antibody

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Anti-CD28 Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-CD28 Antibody
Validated applications
ELISA,FCM
Species reactivity
Reacts with: Human
Specificity
Human CD28
No cross-reactivity in ELISA with
Human PD1
Human CTLA4
Immunogen
Recombinant Human CD28 / TP44 protein (Catalog#11524-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD28 / TP44 (rh CD28 / TP44; Catalog#11524-H08H; P10747-1; Met1-Pro152).
Source
Monoclonal Rabbit IgG Clone #007
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD28 Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution
ELISA 1:5000-1:10000
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD28 Antibody (Rabbit Monoclonal antibody) Images

Flow cytometric analysis of Human CD28 expression on human whole blood lymphocytes. Cells were stained with purified anti-Human CD28, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.

Anti-CD28 Antibody: Alternative Names

Anti-Tp44 Antibody

CD28 Background Information

CD28 (Cluster of Differentiation 28) is a disulphide-bonded glycoprotein belonging to the immunoglobulin (Ig) superfamily, and structurally consists of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. Mouse CD28 is constitutively expressed on the surface of all murine T cells and on developing thymocytes as disulfide-linked homodimers or as monomers. CD28 can binds the B7-1 and B7-2 ligand, and together perform important functions in the T and B cell response pathways. B7/CD28 family members, which can augment or antagonize T-cell receptor signaling, in the regulation of central and peripheral T-cell tolerance. CD28 is thus involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
Full Name
CD28 molecule
References
  • Keir ME, et al. (2005) The B7/CD28 costimulatory family in autoimmunity. Immunol Rev. 204: 128-43.
  • Sansom DM, et al. (2006) The role of CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA-4) in regulatory T-cell biology. Immunol Rev. 212: 131-48.
  • Bjrgo E, et al. (2010) Novel mechanism of signaling by CD28. Immunol Lett. 129(1): 1-6.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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