CD27 Antibody

CD27 Antibody Overview

There are 31 CD27 antibodies which are validated in multiple tissues with various applications, including ELISA, FCM, WB, IHC-P, ICC/IF. There are 9 CD27 antibody for ELISA, 20 CD27 antibody for FCM, 2 CD27 antibody for WB, 3 CD27 antibody for IHC-P, 1 CD27 antibody for ICC/IF. Among all these CD27 antibodies, there are 6 anti-CD27 mouse monoclonal antibodies , 20 anti-CD27 rabbit monoclonal antibodies , 5 anti-CD27 rabbit polyclonal antibodies . All the CD27 anbodies are produced in house and all are in stock. CD27 antibody customerized service is available.

CD27 Antibody ELISA

CD27 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD27 (rh CD27; Catalog#10039-H03H; NP_001233.1; Met 1-Ile 192). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD27 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CD27 Antibody western blot

CD27 Antibody western blot Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD27/TNFRSF7 (rh CD27/TNFRSF7; Catalog#10039-H08B1; P26842; Met1-Ile192).

CD27 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

CD27 Antibody flow cytometry

CD27 Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD27 (rh CD27; Catalog#10039-H03H; NP_001233.1; Met1-Ile192). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD27 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CD27 Antibody immunohistochemistry

CD27 Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Human CD27 (rh CD27; Catalog#10039-H31H; P26842; Met1-Ile192). CD27 specific IgG was purified by Human CD27 affinity chromatography.

CD27 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:1000-1:4000
Notes  

CD27 Antibody ICC / IF

CD27 Antibody ICC / IF Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD27 (rM CD27; Catalog#50110-M08H; NP_001028298.1; Met1-Arg182).

CD27 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes