CD155 / PVR Protein

CD155 / PVR Protein Overview

CD155 / PVR reagents

Entry of the poliovirus into permissive cells is dependent on the expression of a specific receptor, the poliovirus receptor (PVR; 173850), which is an integral membrane glycoprotein. Its extracellular region exhibits 3 immunoglobulin-like domains. Two integral forms, PVR-alpha and PVR-delta, and 2 soluble forms, PVR-beta and PVR-gamma, are lacking a transmembrane region generated by alternative splicing of mRNA. The normal cellular function of PVR is unknown. The identification of human PVR prompted study of its counterpart in other species. In the monkey, 2 genes located at 2 separate genetic loci, AGM1 and AGM2, have been cloned. AGM1 encodes 2 membrane-bound splice variant forms homologous to PVR-alpha and PVR-delta. AGM2 encodes a membrane-bound form, PVR-alpha-2. Morrison and Racaniello (1992) described a murine gene, Mph, as a structural homolog of human PVR. Two Mph molecules, Mph-alpha and Mph-beta, are thought to be generated by alternative splicing from a primary transcript. Following the discovery of the human PVR-related gene (PVRR1; 600644), other PVR-related molecules were sought. Using degenerate oligonucleotides in a PCR-based methodology, Eberle et al. (1995) identified a second PVR-related cDNA, which they called PRR2. Furthermore, they presented evidence that PRR2, and not PVR, is the homolog of Mph, and questioned the existence of a true murine homolog of PVR. The PRR2 gene encodes 2 glycoproteins, PRR2-alpha (short form) and PRR2-delta (long form), which have 69% and 73% identity within Mph-alpha and Mph-beta, respectively. In contrast, the human PVR protein exhibits only 51% identity which is, moreover, restricted to the 3 immunoglobulin domains of the murine protein. Northern blot analysis showed that 2 mRNA isoforms of 3.0 kb (PRR2-alpha) and 4.4 kb (PRR2-delta) are ubiquitously found in various normal human tissues.

CD155 / PVR protein family

Belongs to the nectin family.

CD155 / PVR protein name

Recommended name
Poliovirus receptor
Aliases
CD155, HVED, NECL5, Necl-5, nectin-like 5, Tage4
Alternative name
Nectin-like protein 5 CD_antigen: CD155

CD155 / PVR Gene family protein

CD155 / PVR Protein Sequence

Species Human CD155 / PVR protein
Length 417
Mass (Da) 45303
Sequence Human CD155 / PVR protein sequence
Species
Length
Mass (Da)
Sequence
Species
Length
Mass (Da)
Sequence

CD155 / PVR Protein Molecular Weight & PI

Poliovirus receptor precursor (Nectin-like protein 5) (NECL-5) (CD155 antigen) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 21-417 Poliovirus receptor.

Molecular weight (Da)

43209.93

Theoretical pI

5.94

CD155 / PVR Protein Structure

CryoEM structure of poliovirus receptor bound to poliovirus
Deposited
2003-01-13   Released:  2004-01-27
Deposition Author(s)
He, Y., Mueller, S., Chipman, P.R., Bator, C.M., Peng, X., Bowman, V.D., Mukhopadhyay, S., Wimmer, E., Kuhn, R.J., Rossmann, M.G.
Organism(s)
Poliovirus type 1 (strain Mahoney), Homo sapiens
Expression System
Homo sapiens
Experimental Data Snapshot
Method
ELECTRON MICROSCOPY
1NN8 From PDB

Human CD155 / PVR protein Secondary structure

CD155 / PVR Protein Interaction

Recombinant CD155 / PVR Protein Feature

CD155 / PVR Protein, Human, Recombinant (Fc Tag)

High Purity
> 95 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Measured by its binding ability in a functional ELISA . Immobilized human DNAM1 at 2 μg/ml (100 μl/well) can bind human CD155-Fc with a linear ranger of 0.032-0.8 μg/ml.

Recombinant CD155 / PVR protein citations

Title
CD226 Involves in Immune Synapse
Year
2014
Author
Wei, H;Sun, R;Tian, Z;Hou, S;Ge, K;Zheng, X;
Journal
J. Biol. Chem.
Title
CD226 Involves in Immune Synapse Formation and Triggers NK Cell Activation via Its First Extracellular Domain
Year
2014
Author
Hou, S;Ge, K;Zheng, X;Wei, H;Sun, R;Tian, Z;
Journal
J. Biol. Chem.
Title
CD226 Protein Is Involved in Immune Synapse Formation and Triggers Natural Killer (NK) Cell Activation via Its First Extracellular Domain
Year
2014
Author
S Hou, K Ge, X Zheng, H Wei, R Sun, Z Tian
Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Title
The receptors CD96 and CD226 oppose each other in the regulation of natural killer cell functions
Year
2014
Author
CJ Chan, L Martinet, S Gilfillan…
Journal
Nature immunology
Title
HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors.
Year
2017
Author
Kinosada, H;Yasunaga, JI;Shimura, K;Miyazato, P;Onishi, C;Iyoda, T;Inaba, K;Matsuoka, M;
Journal
PLoS Pathog
Title
TIGIT expression levels on CD4+ T cells are correlated with disease severity in patients with psoriasis
Year
2018
Author
Wang, FF;Wang, Y;Wang, L;Wang, TS;Bai, YP;
Journal
Clin. Exp. Dermatol.

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