IL7R alpha / CD127 Protein, Human, Recombinant (His & Fc Tag)

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IL7R alpha / CD127 Protein, Human, Recombinant (His & Fc Tag): Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
1. Measured by its ability to bind biotinylated mouse TSLP-his (cat:51005-M08H) in functional ELISA.
2. Measured by its binding ability in a functional ELISA. Immobilized human IL7 (Cat:11821-HNAE) at 10 μg/ml (100 μl/well) can bind human IL7Ra-Fch, The EC50 of human IL7Ra-Fch is 15.2-35.6 ng/ml.
Protein Construction
A DNA sequence encoding the human IL7Rα (NP_002176.2) extracellular domain (Met 1-Gly 236) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Glu 21
Molecule Mass
The recombinant human IL7Rα/Fc is a disulfide-linked homodimer. The reduced monomer consists of 464 amino acids and has a predicted molecular mass of 53 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh IL7Rα/Fc monomer is approximately 65-75 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL7R alpha / CD127 Protein, Human, Recombinant (His & Fc Tag): Images

1. Measured by its ability to bind biotinylated mouse TSLP-his (cat:51005-M08H) in functional ELISA. 2. Measured by its binding ability in a functional ELISA. Immobilized human IL7 (Cat:11821-HNAE) at 10 μg/ml (100 μl/well) can bind human IL7Ra-Fch, The EC50 of human IL7Ra-Fch is 15.2-35.6 ng/ml.

IL7R alpha / CD127 Protein, Human, Recombinant (His & Fc Tag): Alternative Names

CD127 Protein, Human; CDW127 Protein, Human; IL-7R Protein, Human; IL-7R-alpha Protein, Human; IL7RA Protein, Human; ILRA Protein, Human

IL7R alpha / CD127 Background Information

Interleukin 7 Receptor alpha (IL-7RA), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival. IL-7 receptor alpha (CD127) signaling is essential for T-cell development and regulation of naive and memory T-cell homeostasis. IL-7RA is critically required for the proper development and function of lymphoid cells. Therefore, the IL-7RA is critically required for the proper development and function of lymphoid cells. Studies from both pathogenic and controlled HIV infection indicate that the containment of immune activation and preservation of CD127 expression are critical to the stability of CD4(+) T cells in infection. A better understanding of the factors regulating CD127 expression in HIV disease, particularly on T(CM) cells, might unveil new approaches exploiting the IL-7/IL-7R receptor pathway to restore T cell homeostasis and promote immune reconstitution in HIV infection. Factors relevant to HIV infection that could potentially decrease CD127 expression on human CD8(+) T cells. CD127 down-regulation may be an important contributor to HIV-associated T-cell dysfunction. In addition to IL-7, IL-7RA also associates with TSLPR to form the functional receptor for thymic stromal lymphopoietin (TSLP) which indirectly regulates T cell development by modulating dendritic cell activation. Mutations in the human IL-7RA gene cause a type of severe combined immunodeficiency in which the major deficiencies are in T cell development, whereas B and NK cells are relatively normal in number. Variation in the IL7RA gene was recently found associated with multiple sclerosis (MS). The polymorphisms in the IL7RA gene is involved in MS pathogenesis and suggest that IL7RA variation may primarily affect chronic disease courses. Soluble CD127 (sCD127) appears to play an important role in the immunopathogenesis of several chronic infections, multiple sclerosis, and various cancers.
Full Name
interleukin 7 receptor
References
  • Vranjkovic A, et al. (2007) IL-7 decreases IL-7 receptor alpha (CD127) expression and induces the shedding of CD127 by human CD8+ T cells. Int Immunol. 19(12): 1329-39.
  • Kiazyk SA, et al. (2008) Loss of CD127 expression links immune activation and CD4(+) T cell loss in HIV infection. Trends Microbiol. 16(12): 567-73.
  • Akkad DA, et al. (2009) Variation in the IL7RA and IL2RA genes in German multiple sclerosis patients. J Autoimmun. 32(2): 110-5.
  • Crawley AM, et al. (2010) Soluble IL-7R alpha (sCD127) inhibits IL-7 activity and is increased in HIV infection. J Immunol. 184(9): 4679-87.
  • ANTIBODIES DIRECTED AGAINST THE ALPHA CHAIN OF IL7 RECEPTOR-THEIR USE FOR THE PREPARATION OF DRUG CANDIDATES
    Author
    S Brouard, LH Mai, C Mary, N Poirier…
    Year
    2014
    Journal
    US Patent …
    Application
    Antigen
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