|Order or Inquire for ANXA5 / Annexin Ⅴ / Annexin A5 Protein (FITC conjugated) product||Quality antibodies||Antibody production services|
ANXA5 / Annexin Ⅴ / Annexin A5 protein are conjugated with FITC under optimum conditions, the unreacted FITC was removed.
5 μl/Test, 0.1 mg/ml
|Formulation :||Aqueous solution containing 0.5% BSA and 0.09% sodium azide|
|Applications :||Flow Cytometry|
|Form & Shipping :||
Liquid. Shipping at ambient temperature.
|Flow Cytometry :||
Flow Cytometric Analysis of FITC Annexin V staining. Jurkat cells were untreated (top panels) or treated for 6 hours with 1 μM Camptothecin (Sigma,Cat.No C9911. bottom panels). Cells were incubated with FITC Annexin V in a buffer containing 7-Amino-Actinomycin (7-AAD,BD Pharmingen™ Cat. No.559925) and analyzed by flow cytometry. Untreated cells were primarily FITC Annexin V and 7-AAD negative, after a 6 hour treatment (bottom panels), there were primarily two populations of cells: Cells that were viable and not undergoing apoptosis (FITC Annexin V and 7-AAD negative); cells undergoing apoptosis (FITC Annexin V positive and 7-AAD negative). A minor population of cells were observed to be FITC Annexin V and 7-AAD positive, indicating that they were in end stage apoptosis or already dead.
This reagent is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
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