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Anti-MICA Antibody

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Anti-MICA Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-MICA Antibody
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Human
Specificity
Human MICA
Immunogen
Recombinant Human MICA protein (Catalog#12302-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human MICA (rh MICA; Catalog#12302-H08H; AAH16929.1; Met 1-Gln 308). MICA specific IgG was purified by Human MICA affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-MICA Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:500-1:2000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-MICA Antibody (Rabbit Polyclonal antibody) Images

Immunochemical staining of human MICA in human breast-carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

MICA was immunoprecipitated using:

Lane A:0.5 mg A431 Whole Cell Lysate

2 µL anti-MICA rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-MICA rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:500 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 55 kDa

Observed band size: 55 kDa

Anti-MICA rabbit polyclonal antibody at 1:500 dilution

Lane A: 293T Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lane C: Daudi Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:43 kDa

Observed band size:55 kDa

Anti-MICA Antibody: Alternative Names

Anti-DAMA-345G11.2 Antibody; Anti-FLJ36918 Antibody; Anti-FLJ60820 Antibody; Anti-MGC111087 Antibody; Anti-MGC21250 Antibody; Anti-MIC-A Antibody; Anti-PERB11.1 Antibody

MICA Background Information

MHC class I chain-related molecules A (MICA) is one of the genes in the HLA class I region, which belongs to MHC class I family. It is the member of the non-classical class I family that displays the greatest degree of polymorphism. The MICA protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. The Natural killer group 2D (NKG2D), a C-type lectin-like activating immunoreceptor, is a receptor of MICA, which was detected on most gammadelta T cells, CD8+ alphabeta T cells, and natural killer (NK) cells. Effector cells from all these subsets could be stimulated by ligation of NKG2D. Engagement of NKG2D activated cytolytic responses of gammadelta T cells and NK cells against transfectants and epithelial tumor cells expressing MICA. The MICA system is a novel, avidin-free immunohistochemical detection system that provides a significant increase in sensitivity compared to traditional immunodetection systems.
Full Name
MHC class I polypeptide-related sequence A
References
  • Choy MK, et al. (2010) MICA polymorphism: biology and importance in immunity and disease. Trends Mol Med. 16(3): 97-106.
  • Li J, et al. (2005) Distinct pattern of human Vdelta1 gammadelta T cells recognizing MICA. Cell Mol Immunol. 2(4): 253-8.
  • Mangham DC, et al. (2000) MICA-a highly sensitive and avidin-free immunohistochemical detection system. Adv Anat Pathol. 7(6): 360-4.
  • Bauer S, et al. (1999) Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science. 285(5428): 727-9.
  • Groh V, et al. (1998) Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells. Science. 279: 1737-40.

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