MICA was immunoprecipitated using:
Lane A:0.5 mg A431 Whole Cell Lysate2 µL anti-MICA rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-MICA rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:
Clean-Blotô IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.
Performed under reducing conditions.Predicted band size: 55 kDa
Observed band size: 55 kDa
Anti-MICA rabbit polyclonal antibody at 1:500 dilution
Lane A: 293T Whole Cell Lysate
Lane B: Jurkat Whole Cell Lysate
Lane C: Daudi Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:43 kDa
Observed band size:55 kDa