Anti-LDLR / LDL Receptor Antibody

1/1
Price:
Size:
Number:

Anti-LDLR / LDL Receptor Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-LDLR / LDL Receptor Antibody
Validated applications
ICC/IF
Species reactivity
Reacts with: Mouse
Specificity
Mouse LDLR / LDL Receptor
Immunogen
Recombinant Mouse LDLR Protein (Catalog#50305-M08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse LDLR (rM LDLR; Catalog#50305-M08H; NP_034830.2; Met1-Arg790).
Source
Monoclonal Rabbit IgG Clone #004
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-LDLR / LDL Receptor Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution
ICC-IF 1:20-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-LDLR / LDL Receptor Antibody (Rabbit Monoclonal antibody) Images

Immunofluorescence staining of mLDLR in RAW264.7 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-mouse mLDLR monoclonal antibody (dilution ratio 1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm .

Anti-LDLR / LDL Receptor Antibody: Alternative Names

Anti-Hlb301 Antibody

LDLR / LDL Receptor Background Information

LDL Receptor, also known as LDLR, is a mosaic protein which belongs to the Low density lipoprotein receptor gene family. The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. LDL Receptor consists of 84 amino acids (after removal of signal peptide) and mediates the endocytosis of cholesterol-rich LDL. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. LDL Receptor is a cell-surface receptor that recognizes the apoprotein B1 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants.
Full Name
low density lipoprotein receptor
References
  • Yamamoto T, et al. (1984) The human LDL receptor: a cysteine-rich protein with multiple Alu sequences in its mRNA. Cell. 39(1): 27-38.
  • Mao B, et al. (2001) LDL-receptor-related protein 6 is a receptor for Dickkopf proteins. Nature. 411(6835): 321-5.
  • Pinson KI, et al. (2000) An LDL-receptor-related protein mediates Wnt signalling in mice. Nature. 407(6803): 535-8.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait