Anti-CD27 Antibody

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Anti-CD27 Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-CD27 Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Human
Specificity
Human CD27
Immunogen
Recombinant Human CD27/TNFRSF7 Protein (Catalog#10039-H08B1)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD27/TNFRSF7 (rh CD27/TNFRSF7; Catalog#10039-H08B1; P26842; Met1-Ile192).
Source
Monoclonal Rabbit IgG Clone #150
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD27 Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD27 Antibody (Rabbit Monoclonal antibody) Images

Anti-CD27 rabbit monoclonal antibody at 1:500 dilution

Lane A: Raji Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:29 kDa

Observed band size:65 kDa

Anti-CD27 Antibody: Alternative Names

Anti-S152 Antibody; Anti-S152. LPFS2 Antibody; Anti-S152.LPFS2 Antibody; Anti-T14 Antibody; Anti-TNFRSF7 Antibody; Anti-Tp55 Antibody

CD27 Background Information

CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has important role in generation of T cell immunity, and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD7 on lymphocytes and dendritic cells. The CD27-CD7 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.
Full Name
CD27 molecule
Research Areas
References
  • Drner T, et al. (2004) Correlation of circulating CD27 high plasma cells and disease activity in systemic lupus erythematosus. Lupus. 13(5): 283-9.
  • Sahota SS, et al. (2009) CD27 in defining memory B-cell origins in Waldenstrm's macroglobulinemia. Clin Lymphoma Myeloma. 9(1): 33-5.
  • Jiang J, et al. (2010) Reduced CD27 expression on antigen-specific CD4+ T cells correlates with persistent active tuberculosis. J Clin Immunol. 30(4): 566-73.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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