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Mouse TPT1 Gene cDNA clone plasmid

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Mouse TPT1 Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:NM_009429.3
Tamanho de cDNA:519bp
Descrição de cDNA:Full length Clone DNA of Mus musculus tumor protein, translationally-controlled 1.
Sinónimo de gene:Trt, p21, p23, TCTP
Espécie:Mouse
Vetor:pGEM-T Vector
Plasmid:pGEM-mTPT1
Local de restrição:
Sequência de etiqueta:
Descrição da sequência:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Mouse TPT1 Gene cDNA clone plasmid on other vectors
Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaMG51648-ACG$225
Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaMG51648-ACR$225
Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-GFPSpark EtiquetaMG51648-ANG$225
Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-OFPSpark EtiquetaMG51648-ANR$225
Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaMG51648-CF$75
Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaMG51648-CH$195
Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaMG51648-CM$195
Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaMG51648-CY$195
Mouse TPT1 Gene cDNA clone plasmidMG51648-G$75
Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaMG51648-NF$195
Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaMG51648-NH$195
Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaMG51648-NM$195
Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaMG51648-NY$195
Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de expressão)MG51648-U$75
Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem)MG51648-UT$195
 Saiba mais sobre vectores de expressão
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Fundo

Tumor protein, also known as TPT1, is a highly conserved protein among many eukaryotic organisms. Tumor protein is involved in a variety of cellular activities, including microtubule stabilization, calcium-binding activities, and apoptosis. The Mammalian translationally controlled tumour protein (TPT1) (or P23) is a protein which has been found to be preferentially synthesised in cells during the early growth phase of some types of tumour, but which is also expressed in normal cells. It was first identified as a histamine-releasing factor, acting in IgE +-dependent allergic reactions. In addition, TPT1 has been shown to bind to tubulin in the cytoskeleton, has a high affinity for calcium, is the binding target for the antimalarial compound artemisinin, and is induced in vitamin D-dependent apoptosis. TPT1 production is thought to be controlled at the translational as well as the transcriptional level.

Referências
  • Thaw P. et al., 2001, Nat Struct Biol. 8 (8): 701-4.
  • Thiele H. et al., 2000, Eur J Biochem. 267 (17): 5473-81.
  • Chitpatima ST. et al., 1988, Nucleic Acids Res. 16 (5): 2350.
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