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Ratazana BAFFR / TNFRSF13C / CD268 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag Etiqueta

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Rat TNFRSF13C Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:XM_576316.3
Tamanho de cDNA:528bp
Descrição de cDNA:Full length Clone DNA of Rattus norvegicus tumor necrosis factor receptor superfamily, member 13c with N terminal Flag tag.
Sinónimo de gene:RGD1560810, Tnfrsf13c
Espécie:Rat
Vetor:pCMV3-SP-N-FLAG
Plasmid:
Local de restrição:
Sequência de etiqueta:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Descrição da sequência:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Ratazana BAFFR / TNFRSF13C / CD268 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag Etiqueta on other vectors
Ratazana BAFFR / TNFRSF13C / CD268 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaRG80171-ACG$225
Ratazana BAFFR / TNFRSF13C / CD268 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaRG80171-ACR$225
Ratazana BAFFR / TNFRSF13C / CD268 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaRG80171-CF$195
Ratazana BAFFR / TNFRSF13C / CD268 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaRG80171-CH$195
Ratazana BAFFR / TNFRSF13C / CD268 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaRG80171-CM$195
Ratazana BAFFR / TNFRSF13C / CD268 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaRG80171-CY$195
Ratazana BAFFR / TNFRSF13C / CD268 clonagem de ADN ou de clonagem do gene (vector de expressão)RG80171-G$75
Ratazana BAFFR / TNFRSF13C / CD268 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaRG80171-NF$195
Ratazana BAFFR / TNFRSF13C / CD268 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaRG80171-NH$195
Ratazana BAFFR / TNFRSF13C / CD268 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaRG80171-NM$195
Ratazana BAFFR / TNFRSF13C / CD268 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaRG80171-NY$195
Ratazana BAFFR / TNFRSF13C / CD268 clonagem de ADN ou de clonagem do gene (vector de clonagem)RG80171-UT$195
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Fundo

Tumor necrosis factor receptor superfamily, member 13C (TNFRSF13C) also known as B-cell-activating factor receptor (BAFFR) and CD268 antigen, is a member of the tumor necrosis factor receptor superfamily. A tumor necrosis factor receptor (TNFR), or death receptor, is a trimeric cytokine receptor that binds tumor necrosis factors (TNF). The receptor cooperates with an adaptor protein which is important in determining the outcome of the response. Members of the TNF receptor superfamily (TNFRSF) have crucial roles in both innate and adaptive immunity and in cellular apoptosis process. Apoptosis is a cell suicide mechanism that enables metazoans to control cell number in tissues and to eliminate individual cells that threaten the animal's survival. Certain cells have unique sensors, termed death receptors or tumour necrosis factor (TNFR), on their surface. Tumour necrosis factors (TNFR) detect the presence of extracellular death signals and, in response, they rapidly ignite the cell's intrinsic apoptosis machinery. It has been proposed that abnormally high levels of BAFFR/TNFRSF13C (CD268) may contribute to the pathogenesis of autoimmune diseases by enhancing the survival of autoreactive B cells.

Referências
  • Ashkenazi A, et al. (1998) Death receptors: signaling and modulation. Science. 281(5381): 1305-8.
  • Losi CG, et al. (2005) Mutational analysis of human BAFF receptor TNFRSF13C (BAFF-R) in patients with common variable immunodeficiency. J Clin Immunol. 25(5): 496-502.
  • Hentges KE, et al. (2002) Tnfrsf13c (Baffr) is mis-expressed in tumors with murine leukemia virus insertions at Lvis22. Genomics. 80(2): 204-12.
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