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Cynomolgus PFK1/PFKM clonagem de ADN ou de clonagem do gene (vector de expressão)

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Cynomolgus PFKM Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:XM_005570698.1
Tamanho de cDNA:2343bp
Descrição de cDNA:Full length Clone DNA of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) phosphofructokinase, muscle.
Sinónimo de gene:PFKM
Espécie:Cynomolgus
Vetor:pGEM-T Vector
Plasmid:pGEM-cynoPFKM
Local de restrição:
Sequência de etiqueta:
Descrição da sequência:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 108T/C, 912G/A not causing the amino acid variation. Please check the sequence information before order.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Cynomolgus PFK1/PFKM clonagem de ADN ou de clonagem do gene (vector de expressão) on other vectors
Cynomolgus PFK1/PFKM clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaCG90449-ACG$245
Cynomolgus PFK1/PFKM clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaCG90449-ACR$245
Cynomolgus PFK1/PFKM clonagem de ADN ou de clonagem do gene (vector de clonagem), N-GFPSpark EtiquetaCG90449-ANG$245
Cynomolgus PFK1/PFKM clonagem de ADN ou de clonagem do gene (vector de clonagem), N-OFPSpark EtiquetaCG90449-ANR$245
Cynomolgus PFK1/PFKM clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaCG90449-CF$215
Cynomolgus PFK1/PFKM clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaCG90449-CH$215
Cynomolgus PFK1/PFKM clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaCG90449-CM$215
Cynomolgus PFK1/PFKM clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaCG90449-CY$215
Cynomolgus PFK1/PFKM clonagem de ADN ou de clonagem do gene (vector de expressão)CG90449-G$75
Cynomolgus PFK1/PFKM clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaCG90449-NF$215
Cynomolgus PFK1/PFKM clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaCG90449-NH$215
Cynomolgus PFK1/PFKM clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaCG90449-NM$215
Cynomolgus PFK1/PFKM clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaCG90449-NY$215
Cynomolgus PFK1/PFKM clonagem de ADN ou de clonagem do gene (vector de clonagem)CG90449-UT$215
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Fundo

PFK1, also known as PFKM, is a regulatory glycolytic enzyme. PFK1 converts fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. It is a muscle-type isozyme. There are three phosphofructokinase isozymes in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Mutations in PFK1 gene have been related with glycogen storage disease type VII, also identified as Tarui disease.

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