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Humano NLGN4X/KIAA1260 clonagem de ADN ou de clonagem do gene (vector de expressão)

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    Humano NLGN4X Informações sobre o produto de clone de cDNA
    Gene_bank_ref_id:NM_020742.2
    Tamanho de cDNA:2451bp
    Descrição de cDNA:Full length Clone DNA of Homo sapiens neuroligin 4, X-linked.
    Sinónimo de gene:HLNX, HNLX, NLGN, HNL4X, NLGN4, ASPGX2, AUTSX2
    Espécie:Human
    Vetor:pGEM-T Vector
    Plasmid:pGEM-NLGN4X
    Local de restrição:
    Sequência de etiqueta:
    Descrição da sequência:Identical with the Gene Bank Ref. ID sequence.
    Sequencing primers:SP6 and T7 or M13-47 and RV-M
    ( We provide with NLGN4X qPCR primers for gene expression analysis, HP104287 )
    Promoter:
    Application:
    Antibiotic in E.coli:Ampicillin
    Antibiotic in mammalian cell:
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
    pGEM-T Vector Information

    The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

    pGEM-T Simple Usage Suggestion:

    The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

    Vector Sequence Download
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