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Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag Etiqueta

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    Rato TPT1 Informações sobre o produto de clone de cDNA
    Gene_bank_ref_id:NM_009429.3
    Tamanho de cDNA:519 bp
    Descrição de cDNA:Full length Clone DNA of Mus musculus tumor protein, translationally-controlled 1
    Sinónimo de gene:p21,p23,TCTP,Trt
    Espécie:Mouse
    Vetor:pCMV3-C-FLAG
    Plasmid:
    Local de restrição:
    Sequência de etiqueta:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
    Descrição da sequência:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with TPT1 qPCR primers for gene expression analysis, MP201521 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Armazenamento:The lyophilized plasmid can be stored at ambient temperature for three months.
    FLAG Tag Info

    FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

    The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

    Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag Etiqueta on other vectors
    Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaMG51648-ACG$225
    Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaMG51648-ACR$225
    Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-GFPSpark EtiquetaMG51648-ANG$225
    Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-OFPSpark EtiquetaMG51648-ANR$225
    Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaMG51648-CF$195
    Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaMG51648-CH$195
    Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaMG51648-CM$195
    Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaMG51648-CY$195
    Mouse TPT1 Gene cDNA clone plasmidMG51648-G$75
    Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaMG51648-NF$195
    Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaMG51648-NH$195
    Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaMG51648-NM$195
    Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaMG51648-NY$195
    Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de expressão)MG51648-U$75
    Ratazanao TCTP /TPT1 clonagem de ADN ou de clonagem do gene (vector de clonagem)MG51648-UT$195
     Saiba mais sobre vectores de expressão
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    Fundo

    Tumor protein, also known as TPT1, is a highly conserved protein among many eukaryotic organisms. Tumor protein is involved in a variety of cellular activities, including microtubule stabilization, calcium-binding activities, and apoptosis. The Mammalian translationally controlled tumour protein (TPT1) (or P23) is a protein which has been found to be preferentially synthesised in cells during the early growth phase of some types of tumour, but which is also expressed in normal cells. It was first identified as a histamine-releasing factor, acting in IgE +-dependent allergic reactions. In addition, TPT1 has been shown to bind to tubulin in the cytoskeleton, has a high affinity for calcium, is the binding target for the antimalarial compound artemisinin, and is induced in vitamin D-dependent apoptosis. TPT1 production is thought to be controlled at the translational as well as the transcriptional level.

    Referências
  • Thaw P. et al., 2001, Nat Struct Biol. 8 (8): 701-4.
  • Thiele H. et al., 2000, Eur J Biochem. 267 (17): 5473-81.
  • Chitpatima ST. et al., 1988, Nucleic Acids Res. 16 (5): 2350.
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    Catálogo: MG51648-CF
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