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Ratazanao CLN2 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA Etiqueta

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Mouse TPP1 Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:NM_009906.5
Tamanho de cDNA:1689bp
Descrição de cDNA:Full length Clone DNA of Mus musculus tripeptidyl peptidase I with N terminal HA tag.
Sinónimo de gene:Cln2; TPP-I
Espécie:Mouse
Vetor:pCMV3-N-HA
Plasmid:
Local de restrição:
Sequência de etiqueta:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Descrição da sequência:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

Ratazanao CLN2 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA Etiqueta on other vectors
Ratazanao CLN2 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaMG52010-ACG$245
Ratazanao CLN2 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaMG52010-ACR$245
Ratazanao CLN2 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-GFPSpark EtiquetaMG52010-ANG$245
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Ratazanao CLN2 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaMG52010-CF$215
Ratazanao CLN2 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaMG52010-CH$215
Ratazanao CLN2 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaMG52010-CM$215
Ratazanao CLN2 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaMG52010-CY$215
Mouse TPP1 Gene cDNA clone plasmidMG52010-G$195
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Ratazanao CLN2 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaMG52010-NM$215
Ratazanao CLN2 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaMG52010-NY$215
Ratazanao CLN2 clonagem de ADN ou de clonagem do gene (vector de expressão)MG52010-U$75
Ratazanao CLN2 clonagem de ADN ou de clonagem do gene (vector de clonagem)MG52010-UT$215
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Fundo

Tripeptidyl-peptidase 1 (TPP1 / CLN2) is a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. TPP1 / CLN2 May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Defects in TPP1 / CLN2 are the cause of neuronal ceroid lipofuscinosis type 2 (CLN2), a form of neuronal ceroid lipofuscinosis which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy.

Referências
  • Xin H, et al. (2007) TPP1 is a homologue of ciliate TEBP-beta and interacts with POT1 to recruit telomerase. Nature. 445(7127): 559-62.
  • O'Connor MS, et al. (2006) A critical role for TPP1 and TIN2 interaction in high-order telomeric complex assembly. Proc Natl Acad Sci U S A. 103(32): 11874-9.
  • Abreu E, et al. (2010) TIN2-tethered TPP1 recruits human telomerase to telomeres in vivo. Mol Cell Biol. 30(12): 2971-82.
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