|Folha de dados||Análises||Produtos relacionados||Protocolos|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse REG4 ORF mammalian expression plasmid, C-GFPSpark tag||MG50202-ACG|
|Mouse REG4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50202-ACR|
|Mouse REG4 ORF mammalian expression plasmid, C-Flag tag||MG50202-CF|
|Mouse REG4 ORF mammalian expression plasmid, C-His tag||MG50202-CH|
|Mouse REG4 ORF mammalian expression plasmid, C-Myc tag||MG50202-CM|
|Mouse REG4 ORF mammalian expression plasmid, C-HA tag||MG50202-CY|
|Mouse REG4 Gene cDNA clone plasmid||MG50202-M|
|Mouse REG4 ORF mammalian expression plasmid, N-Flag tag||MG50202-NF|
|Mouse REG4 ORF mammalian expression plasmid, N-His tag||MG50202-NH|
|Mouse REG4 ORF mammalian expression plasmid, N-Myc tag||MG50202-NM|
|Mouse REG4 ORF mammalian expression plasmid, N-HA tag||MG50202-NY|
|Mouse REG4 natural ORF mammalian expression plasmid||MG50202-UT|
|Saiba mais sobre vectores de expressão|
Regenerating islet-derived protein 4, also known as REG-like protein, REG4, GISP and RELP, a member of the regenerating gene family belonging to the calcium (C-type) dependent lectin superfamily, has been found to be involved in malignancy in several different organs including the stomach, colorectum, pancreas and prostate. It is highly expressed in the gastrointestinal tract and markedly up-regulated in colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, and inflammatory bowel disease. Expression of the Reg4 in different cell types has been associated with regeneration, cell growth and cell survival, cell adhesion and resistance to apoptosis. REG4 protein overexpression is associated with an unfavorable response to preoperative chemoradiotherapy and may be used as a predictive biomarker clinically. REG4 may play an important role in the development and progression of colorectal cancer, as well as in intestinal morphogenesis and epithelium restitution.