PRMT6 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag General Information
Full length Clone DNA of Mouse protein arginine N-methyltransferase 6 with N terminal Flag tag.
Enhanced CMV promoter
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
The plasmid is confirmed by full-length sequencing.
Antibiotic in E.coli
Antibiotic in Mammalian cell
Stable or Transient mammalian expression
Storage & Shipping
Each tube contains lyophilized plasmid.
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
PRMT6 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag Alternative Names
AW124876 cDNA ORF Clone, Mouse;BB233495 cDNA ORF Clone, Mouse;Hrmt1l6 cDNA ORF Clone, Mouse
PRMT6 Background Information
Protein arginine N-methyltransferase 6, also known as Histone-arginine N-methyltransferase PRMT6, PRMT6, and HRMT1L6, is a member of the protein arginine N-methyltransferase family and PRMT6 subfamily. PRMT6 is highly expressed in kidney and testes. PRMT6 is known to catalyze the generation of asymmetric dimethylarginine in polypeptides. It has been implicated in human immunodeficiency virus pathogenesis, DNA repair, and transcriptional regulation. PRMT6 is known to methylate histone H3 Arg-2 (H3R2), and this negatively regulates the lysine methylation of H3K4 resulting in gene repression. PRMT6 plays a key role in coupling process by functioning as a transcriptional coactivator that can regulate alternative splicing. PRMT6 coactivates the progesterone, glucocorticoid and oestrogen receptors in luciferase reporter assays in a hormone-dependent manner. Small interfering RNA (siRNA) oligonucleotide duplex knockdown of PRMT6 disrupts oestrogen-stimulated transcription of endogenous GREB1 and progesterone receptor in MCF-7 breast cancer cells. Neutralizing the activity of PRMT6 could inhibit tumor progression and may be of cancer therapeutic significance.
protein arginine methyltransferase 6
Hyllus D, et al., 2007, Genes Dev. 21(24): 3369-80. Lakowski, TM. et al., 2008, J Biol Chem. 283 (15): 10015-25. Michaud-Levesque, J. et al., 2009, J Biol Chem. 284 (32): 21338-46. Harrison, MJ. et al., 2010, Nucleic acids Res. Jan 4.