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Ratazanao Peroxiredoxin 1/PRDX1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA Etiqueta

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Mouse PRDX1 Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:NM_011034.4
Tamanho de cDNA:600bp
Descrição de cDNA:Full length Clone DNA of Mus musculus peroxiredoxin 1 with N terminal HA tag.
Sinónimo de gene:PAG, OSF3, Paga, PrxI, TDX2, TPxA, prx1, MSP23, NkefA, OSF-3, PrdxI, Tdpx2, Prdx1
Espécie:Mouse
Vetor:pCMV3-N-HA
Plasmid:
Local de restrição:
Sequência de etiqueta:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Descrição da sequência:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

Ratazanao Peroxiredoxin 1/PRDX1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA Etiqueta on other vectors
Ratazanao Peroxiredoxin 1/PRDX1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaMG50552-ACG$225
Ratazanao Peroxiredoxin 1/PRDX1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaMG50552-ACR$225
Ratazanao Peroxiredoxin 1/PRDX1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-GFPSpark EtiquetaMG50552-ANG$225
Ratazanao Peroxiredoxin 1/PRDX1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-OFPSpark EtiquetaMG50552-ANR$225
Ratazanao Peroxiredoxin 1/PRDX1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaMG50552-CF$195
Ratazanao Peroxiredoxin 1/PRDX1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaMG50552-CH$195
Ratazanao Peroxiredoxin 1/PRDX1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaMG50552-CM$195
Ratazanao Peroxiredoxin 1/PRDX1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaMG50552-CY$195
Ratazanao Peroxiredoxin 1/PRDX1 clonagem de ADN ou de clonagem do gene (vector de expressão)MG50552-M$75
Ratazanao Peroxiredoxin 1/PRDX1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaMG50552-NF$195
Ratazanao Peroxiredoxin 1/PRDX1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaMG50552-NH$195
Ratazanao Peroxiredoxin 1/PRDX1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaMG50552-NM$195
Ratazanao Peroxiredoxin 1/PRDX1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaMG50552-NY$195
Ratazanao Peroxiredoxin 1/PRDX1 clonagem de ADN ou de clonagem do gene (vector de clonagem)MG50552-UT$195
 Saiba mais sobre vectores de expressão
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Fundo

Peroxiredoxin-1, also known as Thioredoxin peroxidase 2, Natural killer cell-enhancing factor A, PRDX1, and PAGA, is a member of the ahpC/TSA family. Peroxiredoxin-1 is constitutively expressed in most human cells. It is induced to higher levels upon serum stimulation in untransformed and transformed cells. Peroxiredoxins (PRDXs) are a family of antioxidant enzymes that are also known as scavengers of peroxide in mammalian cells. The overexpression of Peroxiredoxin-1, which is one of the peroxiredoxins that is a ubiquitously expressed protein, was related to a poor prognosis in several types of human cancers. Peroxiredoxin-1 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin and may play an important role in eliminating peroxides generated during metabolism. Peroxiredoxin-1 Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. The reduced Peroxiredoxin-1 expression is an important factor in esophageal squamous cancer progression and could serve as a useful prognostic marker.

Referências
  • Neumann, CA. et al., 2003, Nature 424 (6948): 561-5
  • Gisin, J. et al., 2005, J Clin Pathol. 58 (11): 1229-31.
  • Hoshino, I. et al., 2007, Oncol Rep. 18 (4): 867-71.
  • Cao, J. et al., 2009, EMBO J. 28 (10): 1505-17.
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