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Ratazanao ITK Kinase clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc Etiqueta

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Mouse ITK Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:NM_010583.2
Tamanho de cDNA:1860bp
Descrição de cDNA:Full length Clone DNA of Mus musculus IL2-inducible T-cell kinase with C terminal Myc tag.
Sinónimo de gene:Emt, Tsk, Tcsk, Itk
Espécie:Mouse
Vetor:pCMV3-C-Myc
Plasmid:
Local de restrição:
Sequência de etiqueta:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Descrição da sequência:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Ratazanao ITK Kinase clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc Etiqueta on other vectors
Ratazanao ITK Kinase clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaMG50361-ACG$245
Ratazanao ITK Kinase clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaMG50361-ACR$245
Ratazanao ITK Kinase clonagem de ADN ou de clonagem do gene (vector de clonagem), N-GFPSpark EtiquetaMG50361-ANG$245
Ratazanao ITK Kinase clonagem de ADN ou de clonagem do gene (vector de clonagem), N-OFPSpark EtiquetaMG50361-ANR$245
Ratazanao ITK Kinase clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaMG50361-CF$215
Ratazanao ITK Kinase clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaMG50361-CH$215
Ratazanao ITK Kinase clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaMG50361-CM$215
Ratazanao ITK Kinase clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaMG50361-CY$215
Ratazanao ITK Kinase clonagem de ADN ou de clonagem do gene (vector de expressão)MG50361-M$75
Ratazanao ITK Kinase clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaMG50361-NF$215
Ratazanao ITK Kinase clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaMG50361-NH$215
Ratazanao ITK Kinase clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaMG50361-NM$215
Ratazanao ITK Kinase clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaMG50361-NY$215
Ratazanao ITK Kinase clonagem de ADN ou de clonagem do gene (vector de clonagem)MG50361-UT$215
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Fundo

IL-2-inducible T cell kinase is a member of the protein kinase superfamily, Tyr protein kinase family and TEC subfamily. It contains 1 Btk-type zinc finger, 1 PH domain, 1 protein kinase domain, 1 SH2 domain and 1 SH3 domain. As an intracellular kinase which expressed in T-cells, IL-2-inducible T cell kinase contains both SH2 and SH3 domains which are often found in intracellular kinases. It is hought to play a role in T-cell proliferation and differentiation. It regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. IL-2-inducible T cell kinase also plays an essential role in regulation of the adaptive immune response. efects in IL-2-inducible T cell kinase are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1). LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma.

Referências
  • Lee SH, et al. (2011) The association of a single-nucleotide polymorphism of the IL-2 inducible T-cell Kinase gene with asthma. Ann Hum Genet. 75(3):359-69.
  • Yao HL, et al. (2010) Effect of Itk down regulation on cytokines production in Jurkat cell. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 24(5):358-61.
  • Pechloff K, et al. (2010) The fusion kinase ITK-SYK mimics a T cell receptor signal and drives oncogenesis in conditional mouse models of peripheral T cell lymphoma. J Exp Med. 207(5):1031-44.
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