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Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de expressão)

Folha de dadosAnálisesProdutos relacionadosProtocolos
Mouse HAPLN1 Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:AF139572.1
Tamanho de cDNA:1071bp
Descrição de cDNA:Full length Clone DNA of Mus musculus hyaluronan and proteoglycan link protein 1.
Sinónimo de gene:LP, CLP, LP-1, Crtl1, Crtl1l, BB099155
Espécie:Mouse
Vetor:PGEM-T Vector
Plasmid:pGEM-mHAPLN1
Local de restrição:
Sequência de etiqueta:
Descrição da sequência:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 804T>C and 987T>G not causing the amino acid variation.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de expressão) on other vectors
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaMG52939-ACG$225
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaMG52939-ACR$225
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-GFPSpark EtiquetaMG52939-ANG$225
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-OFPSpark EtiquetaMG52939-ANR$225
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaMG52939-CF$195
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaMG52939-CH$195
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaMG52939-CM$195
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaMG52939-CY$195
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de expressão)MG52939-G$75
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaMG52939-NF$195
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaMG52939-NH$195
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaMG52939-NM$195
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaMG52939-NY$195
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem)MG52939-UT$195
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Fundo

Hyaluronan (HA) is a high MW glycosaminoglycan significantly involved in the formation and stability of extracellular matrix via its association with specific HA-binding proteins. HAPLN1, also known as CRTL1 (Cartilage Link Protein 1, cLP ) and link protein, is a member of HA-binding protein (hyaladherins) family, and contains a common structural domain of about 100 amino acids that is termed a Link module with two α-helices and two antiparallel β-sheets. HAPLN1/CRTL1 stabilizes the interaction between hyaluronan (HA) and versican, two extracellular matrix components essential for cardiac development. Link module superfamily can be divided into three subgroups, and the HAPLN family are C domain-type proteins that have an extended structure with one N-terminal V-type Ig-like domain followed by two link modules. In cartilage, aggrecan forms - cLP stabilized aggregates with HA that provides the tissue with its load bearing properties. HAPLN1 is a component of follicular matrix, was shown to enhance cumulus-oocyte complex (COC) expansion in vitro. HAPLN1 may promote periovulatory granulosa cell survival, which would facilitate their differentiation into luteal cells.

Referências
  • Sun GW, et al. (2003) Follicle-stimulating hormone and insulin-like growth factor I synergistically induce up-regulation of cartilage link protein (Crtl1) via activation of phosphatidylinositol-dependent kinase/Akt in rat granulosa cells. Endocrinology. 144(3): 793-801.
  • Wirrig EE, et al. (2007) Cartilage link protein 1 (Crtl1), an extracellular matrix component playing an important role in heart development. Dev Biol. 310(2): 291-303.
  • Liu J, et al. (2010) Periovulatory expression of hyaluronan and proteoglycan link protein 1 (Hapln1) in the rat ovary: hormonal regulation and potential function. Mol Endocrinol. 24(6): 1203-17.
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