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Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag Etiqueta

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Mouse HAPLN1 Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:AF139572.1
Tamanho de cDNA:1071bp
Descrição de cDNA:Full length Clone DNA of Mus musculus hyaluronan and proteoglycan link protein 1 with N terminal Flag tag.
Sinónimo de gene:LP, CLP, LP-1, Crtl1, Crtl1l, BB099155
Espécie:Mouse
Vetor:pCMV3-N-FLAG
Plasmid:
Local de restrição:
Sequência de etiqueta:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Descrição da sequência:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag Etiqueta on other vectors
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaMG52939-ACG$225
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaMG52939-ACR$225
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-GFPSpark EtiquetaMG52939-ANG$225
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-OFPSpark EtiquetaMG52939-ANR$225
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaMG52939-CF$195
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaMG52939-CH$195
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaMG52939-CM$195
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaMG52939-CY$195
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de expressão)MG52939-G$75
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaMG52939-NF$195
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaMG52939-NH$195
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaMG52939-NM$195
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaMG52939-NY$195
Ratazanao HAPLN1 clonagem de ADN ou de clonagem do gene (vector de clonagem)MG52939-UT$195
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Fundo

Hyaluronan (HA) is a high MW glycosaminoglycan significantly involved in the formation and stability of extracellular matrix via its association with specific HA-binding proteins. HAPLN1, also known as CRTL1 (Cartilage Link Protein 1, cLP ) and link protein, is a member of HA-binding protein (hyaladherins) family, and contains a common structural domain of about 100 amino acids that is termed a Link module with two α-helices and two antiparallel β-sheets. HAPLN1/CRTL1 stabilizes the interaction between hyaluronan (HA) and versican, two extracellular matrix components essential for cardiac development. Link module superfamily can be divided into three subgroups, and the HAPLN family are C domain-type proteins that have an extended structure with one N-terminal V-type Ig-like domain followed by two link modules. In cartilage, aggrecan forms - cLP stabilized aggregates with HA that provides the tissue with its load bearing properties. HAPLN1 is a component of follicular matrix, was shown to enhance cumulus-oocyte complex (COC) expansion in vitro. HAPLN1 may promote periovulatory granulosa cell survival, which would facilitate their differentiation into luteal cells.

Referências
  • Sun GW, et al. (2003) Follicle-stimulating hormone and insulin-like growth factor I synergistically induce up-regulation of cartilage link protein (Crtl1) via activation of phosphatidylinositol-dependent kinase/Akt in rat granulosa cells. Endocrinology. 144(3): 793-801.
  • Wirrig EE, et al. (2007) Cartilage link protein 1 (Crtl1), an extracellular matrix component playing an important role in heart development. Dev Biol. 310(2): 291-303.
  • Liu J, et al. (2010) Periovulatory expression of hyaluronan and proteoglycan link protein 1 (Hapln1) in the rat ovary: hormonal regulation and potential function. Mol Endocrinol. 24(6): 1203-17.
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