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Ratazanao RACK1/GNB2L1 clonagem de ADN ou de clonagem do gene (vector de expressão)

Folha de dadosAnálisesProdutos relacionadosProtocolos
Mouse GNB2L1 Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:NM_008143.3
Tamanho de cDNA:954bp
Descrição de cDNA:Full length Clone DNA of Mus musculus guanine nucleotide binding protein (G protein), beta polypeptide 2 like 1.
Sinónimo de gene:p205, Rack1, GB-like, AL033335, Gnb2-rs1
Espécie:Mouse
Vetor:PGEM-T Vector
Plasmid:pGEM-mGNB2L1
Local de restrição:
Sequência de etiqueta:
Descrição da sequência:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Ratazanao RACK1/GNB2L1 clonagem de ADN ou de clonagem do gene (vector de expressão) on other vectors
Ratazanao RACK1/GNB2L1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaMG52808-ACG$225
Ratazanao RACK1/GNB2L1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaMG52808-ACR$225
Ratazanao RACK1/GNB2L1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-GFPSpark EtiquetaMG52808-ANG$225
Ratazanao RACK1/GNB2L1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-OFPSpark EtiquetaMG52808-ANR$225
Ratazanao RACK1/GNB2L1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaMG52808-CF$195
Ratazanao RACK1/GNB2L1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaMG52808-CH$195
Ratazanao RACK1/GNB2L1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaMG52808-CM$195
Ratazanao RACK1/GNB2L1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaMG52808-CY$195
Ratazanao RACK1/GNB2L1 clonagem de ADN ou de clonagem do gene (vector de expressão)MG52808-G$75
Ratazanao RACK1/GNB2L1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaMG52808-NF$195
Ratazanao RACK1/GNB2L1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaMG52808-NH$195
Ratazanao RACK1/GNB2L1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaMG52808-NM$195
Ratazanao RACK1/GNB2L1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaMG52808-NY$195
Ratazanao RACK1/GNB2L1 clonagem de ADN ou de clonagem do gene (vector de clonagem)MG52808-UT$195
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Fundo

RACK1 (guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1), also known as GNB2L1, contains 7 WD repeats and belongs to the WD repeat G protein beta family. In the liver, RACK1 is expressed at higher levels in activated hepatic stellate cells than in hepatocytes or Kupffer cells. It is up-regulated in hepatocellular carcinomas and in the adjacent non-tumor liver tissue. RACK1 is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. It interacts with a wide variety of proteins and plays a role in many cellular processes. GNB2L1 binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. RACK1 may recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. It inhibits the activity of SRC kinases including SRC, LCK and YES1. RACK1 also inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. It enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer.

Referências
  • Jannot G, et al.. (2011) The ribosomal protein RACK1 is required for microRNA function in both C. elegans and humans. EMBO Rep. 12(6):581-6.
  • Wang F, et al. (2011) RACK1 regulates VEGF/Flt1-mediated cell migration via activation of a PI3K/Akt pathway. J Biol Chem. 286(11):9097-106.
  • Cao XX, et al. (2011) RACK1 promotes breast carcinoma migration/metastasis via activation of the RhoA/Rho kinase pathway. Breast Cancer Res Treat. 126(3):555-63.
  • Myklebust LM, et al. (2011) Receptor for activated protein C kinase 1 (RACK1) is overexpressed in papillary thyroid carcinoma. Thyroid. 21(11):1217-25.
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