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Ratazanao GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA Etiqueta

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Mouse GAPDH Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:NM_008084.2
Tamanho de cDNA:1002bp
Descrição de cDNA:Full length Clone DNA of Mus musculus glyceraldehyde-3-phosphate dehydrogenase with N terminal HA tag.
Sinónimo de gene:Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, Gapdh
Espécie:Mouse
Vetor:pCMV3-N-HA
Plasmid:
Local de restrição:
Sequência de etiqueta:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Descrição da sequência:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

Ratazanao GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA Etiqueta on other vectors
Ratazanao GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaMG50545-ACG$225
Ratazanao GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaMG50545-ACR$225
Ratazanao GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), N-GFPSpark EtiquetaMG50545-ANG$225
Ratazanao GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), N-OFPSpark EtiquetaMG50545-ANR$225
Ratazanao GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaMG50545-CF$195
Ratazanao GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaMG50545-CH$195
Ratazanao GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaMG50545-CM$195
Ratazanao GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaMG50545-CY$195
Ratazanao GAPDH clonagem de ADN ou de clonagem do gene (vector de expressão)MG50545-M$75
Ratazanao GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaMG50545-NF$195
Ratazanao GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaMG50545-NH$195
Ratazanao GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaMG50545-NM$195
Ratazanao GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaMG50545-NY$195
Ratazanao GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem)MG50545-UT$195
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Fundo

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.

Referências
  • Hara MR, et al. (2006) Neuroprotection by pharmacologic blockade of the GAPDH death cascade. PNA. 103 (10): 3887-9.
  • Hara MR, et al. (2006) GAPDH as a sensor of NO stress.Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1762 (5): 502-9.
  • Tarze A, et al. (2007) GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilizationGAPDH and apoptosis. Oncogene. 26: 2606-20.
  • Yi MK, et al. (2000) Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function. Journal of Virology. 74 (14) : 6459-68.
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    Catálogo: MG50545-NY
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    Disponibilidade2-3 weeks
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