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Ratazanao ATP5D clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag Etiqueta

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Mouse ATP5D Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:NM_025313.2
Tamanho de cDNA:507bp
Descrição de cDNA:Full length Clone DNA of Mus musculus ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit with C terminal Flag tag.
Sinónimo de gene:C85518, AA960090, AI876556, AU020773, 0610008F14Rik, 1500000I11Rik
Espécie:Mouse
Vetor:pCMV3-C-FLAG
Plasmid:
Local de restrição:
Sequência de etiqueta:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Descrição da sequência:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Ratazanao ATP5D clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag Etiqueta on other vectors
Ratazanao ATP5D clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaMG52204-ACG$225
Ratazanao ATP5D clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaMG52204-ACR$225
Ratazanao ATP5D clonagem de ADN ou de clonagem do gene (vector de clonagem), N-GFPSpark EtiquetaMG52204-ANG$225
Ratazanao ATP5D clonagem de ADN ou de clonagem do gene (vector de clonagem), N-OFPSpark EtiquetaMG52204-ANR$225
Ratazanao ATP5D clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaMG52204-CF$195
Ratazanao ATP5D clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaMG52204-CH$195
Ratazanao ATP5D clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaMG52204-CM$195
Ratazanao ATP5D clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaMG52204-CY$195
Ratazanao ATP5D clonagem de ADN ou de clonagem do gene (vector de expressão)MG52204-G$75
Ratazanao ATP5D clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaMG52204-NF$195
Ratazanao ATP5D clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaMG52204-NH$195
Ratazanao ATP5D clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaMG52204-NM$195
Ratazanao ATP5D clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaMG52204-NY$195
Ratazanao ATP5D clonagem de ADN ou de clonagem do gene (vector de clonagem)MG52204-UT$195
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Fundo

ATP5D is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase consists of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5D gene encodes the delta subunit of the catalytic core.

Referências
  • Jordan EM. et al., 1992, Biochim Biophys Acta 1130 (1): 123-6.
  • Yoshida M. et al., 2001, Nat Rev Mol Cell Biol. 2 (9): 669-77.
  • Hochstrasser DF. et al., 1993, Electrophoresis. 13 (12): 992-1001.
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